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Author:
Date:
2014-07-20
[Abstract] Antimicrobial peptides are known to disrupt bacterial membranes allowing solutes to flow across the membrane in an unregulated manner resulting in death of the organism. Disrupting the bacterial membrane would thus perturb the cells osmotic balance resulting in an initial influx of the external aqueous buffer. We have designed an assay to investigate how antimicrobial peptide concentration affects the ability of fluorescently labelled dextran moieties of differing molecular weight and hydrodynamic radii to cross membranes of viable bacteria. This assay was used to show that diffusion of low and high molecular weight dextrans into bacteria was a function of antimicrobial peptide concentration (Sani et al., 2013).
[摘要] 已知抗微生物肽破坏细菌膜，允许溶质以不受调节的方式流过膜，导致生物体死亡。 破坏细菌膜将因此干扰细胞渗透平衡，导致外部水性缓冲液的初始流入。 我们设计了一种测定，以研究抗微生物肽浓度如何影响不同分子量和流体动力学半径的荧光标记的葡聚糖部分穿过活细菌的膜的能力。 该试验用于显示低分子量和高分子量葡聚糖在细菌中的扩散是抗微生物肽浓度的函数（Sani等人，2013）。
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