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M50 Super 8x TOPFlash Plasmid sent as bacteria in agar stab

M50 Super 8x TOPFlash质粒作为细菌在琼脂刺中发送

Company: Addgene
Catalog#: 12456
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Wnt Reporter Activity Assay
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2014-07-20
[Abstract]  This protocol is for testing responses of a candidate cell line/cell lines to Wnt ligands or Wnt pathway agonists stimulation. This protocol can also be adapted to screen small molecule libraries or biologics that contain activities to either increase or decrease Wnt pathway responses. Canonical Wnt signaling activity transcriptionally induces Wnt target genes that contain concensus TCF/LEF binding element. Wnt pathway activity responsive cells transiently or stably expressing luciferase proteins under the TCF/LEF promoter element can be used to report stimulus-dependent Wnt-pathway activity. We acquired the TopFlash (TCL/LEF-Firefly luciferase) construct from Addgene. [摘要]  该方案用于测试候选细胞系/细胞系对Wnt配体或Wnt通路激动剂刺激的反应。 该方案还可以适于筛选含有增加或降低Wnt途径反应的活性的小分子文库或生物制品。 规范Wnt信号活动转录诱导含有概括TCF/LEF结合元件的Wnt靶基因。 在TCF/LEF启动子元件下瞬时或稳定表达荧光素酶蛋白的Wnt途径活性应答性细胞可用于报告刺激依赖性Wnt通路活性。 我们从Addgene获得TopFlash(TCL/LEF-Firefly萤光素酶)构建体。

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