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µ-Dish 35 mm, high Glass Bottom

35mm培养皿,带玻璃底或8孔微孔板

Company: ibidi
Catalog#: 81158
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Phototaxis Assays of Synechocystis sp. PCC 6803 at Macroscopic and Microscopic Scales
Author:
Date:
2017-06-05
[Abstract]  Phototaxis is a mechanism that allows cyanobacteria to respond to fluctuations in the quality and quantity of illumination by moving either towards or away from a light source. Phototactic movement on low concentration agar or agarose plates can be analyzed at macroscopic and microscopic scales representing group behavior and single cell motility, respectively. Here, we describe a detailed procedure for phototaxis assays on both scales using the unicellular cyanobacterium Synechocystis sp. PCC 6803. [摘要]  Phototaxis是允许蓝细菌通过朝向或远离光源移动来响应照明质量和数量的波动的机制。分别在低浓度琼脂或琼脂糖平板上的光镜移动可以分别表示组织行为和单细胞运动性的宏观和微观尺度。在这里,我们描述了使用单细胞蓝细菌集胞藻的两种鳞片上的光趋化测定的详细程序。 PCC 6803。

背景 模型生物 Synechocystis sp。 PCC 6803使用伸缩型IV皮脂(T4P)以潮汐表面移动,称为抽动动力。两个分泌的ATP酶(PilB和PilT)负责扩增和缩回菌毛装置,从而将细胞向前拉。集胞藻 sp。 PCC 6803拥有覆盖整个可见光谱的各种光感受器。光的吸收可以根据波长和强度刺激正或负光趋向性。最近,已经证明,单细胞集胞藻 PCC 6803能够通过将光聚焦在远侧的尖锐焦点上来直接检测单向照明(Schuergers等人,2016)。此外,显示抽动运动的方向与运动ATPase PilB的特定近端定位相关(Schuergers等人,2015)。提出了一种模型,即聚焦导致对运动装置的局部抑制,从而确定单个细胞作为远离焦点的光电响应的移动方向(Schuergers等人,2016) )。

Three Dimensional Spheroid Co-culture Invasion Assay
Author:
Date:
2015-01-05
[Abstract]  The assay was developed to investigate the impact of stromal cells of different types (in our case breast cancer associated fibroblasts stably manipulated to modify expression of genes of interest) on the invasive capacity of epithelial cancer cells (in our case breast cancer cell lines) (Verghese et al., 2013). Typical two dimensional invasion assays do necessarily account for the presence of extracellular matrix that is present around the stromal and tumour cells in vivo and therefore cellular behaviour within these cultures may be non-physiological. This spheroid assay was developed to attempt to replicate more closely the environment that is present around breast cancer stromal and tumour cells in actual tumours (Verghese et al., 2013). Extra cellular ... [摘要]  开发该测定以研究不同类型(在我们的情况下乳腺癌相关的成纤维细胞稳定操纵以修饰目的基因的表达)的基质细胞对上皮癌细胞(在我们的乳腺癌细胞系)的侵袭能力的影响( Verghese ,,2013)。典型的二维侵袭测定确实需要考虑在体内基质和肿瘤细胞周围存在的细胞外基质的存在,因此这些培养物中的细胞行为可能是非生理的。开发这种球状体测定以尝试更密切地复制存在于实际肿瘤中的乳腺癌基质和肿瘤细胞周围的环境(Verghese等人,2013)。包括由胶原IV和胶原I组成的细胞外基质,并且成纤维细胞和上皮细胞被给予发展"生理"相互作用的机会(Verghese等人,2013; Hooper等人, ,2006)。该方法由Nowicki等人(2008)开发,并且在Verghese等人(2013)中使用它来公开数据。

Design of a Transcription-based Secretion Activity Reporter (TSAR) for the Type III Secretion Apparatus of Shigella flexneri and Uses Thereof
Author:
Date:
2014-10-20
[Abstract]  Many gram-negative bacterial pathogens, including Shigella flexneri, are able to translocate bacterial proteins, dubbed effectors, across the host cell plasma membrane into the host cell cytosol using a syringe-like structure, the type three secretion apparatus (T3SA). While some bacteria use their T3SA to modulate their phagosomal environment (Salmonella spp.), establish pedestal structure to form microcolonies on the plasma membrane (Enteropathogenic Escherichi coli) or lyse their entry vacuole (Shigella spp.), they all have in common a tightly regulated activity of their T3SA. However, the tracking of the activity of the T3SA in infected cells and tissue has been difficult to perform. Using the property of MxiE-dependent promoters that are ... [摘要]  包括灵芝氏菌在内的许多革兰氏阴性细菌病原体能够使用注射器样结构将类型三分泌物穿过宿主细胞质膜转运细菌蛋白质(配体效应子)到宿主细胞胞质溶胶中装置(T3SA)。虽然一些细菌使用它们的T3SA调节它们的吞噬体环境(沙门氏菌

spp 。),建立基质结构以在质膜上形成微集落(Enteropathogenic < em="">)或溶解它们的进入泡(志贺氏菌属。),它们都具有共同的严格调节的他们的T3SA的活性。然而,T3SA在感染的细胞和组织中的活性的跟踪一直难以进行。使用在T3SA是活性时上调的MxiE依赖性启动子的性质,我们最近设计了基于转录的分泌活性报道分子(TSAR),其允许以下的S的活性。使用快速成熟的GFP内在荧光,在组织培养细胞中实时地和体内实时分析灵敏度。在这里我们描述TSAR的设计及其应用于固定和活体样品的显微镜和流式细胞仪在结肠上皮细胞模型使用TC7组织培养细胞。

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