| Adoptive Transfer of Myeloid-Derived Suppressor Cells and T Cells in a Prostate Cancer Model
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Author:
Date:
2015-08-20
[Abstract] The adoptive transfer of immune cells for cancer, chronic infection, and autoimmunity is an emerging field that has shown promise in recent trials. The transgenic adenocarcinoma mouse prostate (TRAMP) is a classical mouse model of prostate cancer (PCa) and TRAMP cell lines were derived from a TRAMP mouse tumor. TRAMP-C2 is tumorigenic when (subcutaneously) s.c. grafted into syngeneic C57BL/6 host mice (Foster et al., 1997). This protocol will describe the adoptive transfer of purified CD11b+Gr1+ double positive (DP) myeloid-derived suppressor cells (MDSC) and CD3+ T cells in the TRAMP-C2 prostate cancer mouse model in order to establish the intrinsic functionality of these immune cells and to determine their role in tumorigenesis in vivo ...
[摘要] 免疫细胞用于癌症，慢性感染和自身免疫的过继转移是一个新兴领域，其在最近的试验中显示出希望。 转基因腺癌小鼠前列腺（TRAMP）是前列腺癌（PCa）的经典小鼠模型，TRAMP细胞系衍生自TRAMP小鼠肿瘤。 当皮下注射时，TRAMP-C2是致瘤的。 移植到同基因C57BL/6宿主小鼠中（Foster等人，1997）。 该方案将描述纯化的CD11b +（Ad）双重阳性（DP）骨髓衍生的抑制细胞（MDSC）和CD3 + sup/+的过继转移。 T细胞在TRAMP-C2前列腺癌小鼠模型中的表达，以便建立这些免疫细胞的内在功能并且确定它们在体内的肿瘤发生中的作用（Yan等人）。 ，2014）。
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| Competitive Bone-marrow Transplantations
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Author:
Date:
2014-06-05
[Abstract] Competitive bone marrow transplantation assay measures multi-lineage reconstitution of hematopoiesis in irradiated transplant recipient mice. Thus this assay is routinely used to determine haematopoietic stem and progenitor cells (HSPCs) functionality in vivo. The principle of the method is to transplant bone marrow donor cells (derived from transgenic mice of choice) on C57BL6 background together with normal competitor bone marrow. In order to distinguish donor from competitor cells upon transplantation, usually competitor mice are congenic and carry the differential B cell antigen originally designated Ly5.1 and CD45.1.
A typical competitive bone marrow transplantation experiment will contain two transplantation groups, donor (transgenic mice of choice and their ...
[摘要] 竞争性骨髓移植测定测量照射的移植受体小鼠中造血的多谱系重建。 因此，该测定常规用于在体内确定造血干细胞和祖细胞（HSPC）功能。 该方法的原理是将骨髓供体细胞（源自选择的转基因小鼠）与正常竞争剂骨髓一起移植到C57BL6背景上。 为了在移植时区分供体和竞争者细胞，通常竞争性小鼠是同基因的并携带最初称为Ly5.1和CD45.1的差别B细胞抗原。典型的竞争性骨髓移植实验将含有两个移植组， 供体（选择的转基因小鼠及其对照）与正常竞争剂竞争移植，并在血液和骨髓中评价移入效率。
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