| Cell Adhesion Assay
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Author:
Date:
2012-03-05
[Abstract] Cell adhesion, the binding of a cell to the extracellular matrix (ECM), other cells, or a specific surface, is essential for the growth and survival of the cell and also its communication with other cells. The process of cell adhesion involves a range of biological events such as three-dimensional re-organization of the cytoskeleton, biochemical reactions in the cell, and changes in molecules on the surface of the cell. Cancer cells, especially the highly metastatic types, are believed to have enhanced adhesion ability that often facilitates the migration of the cells to a new site to establish new tumors in the body. Cell adhesion assay is therefore often used to evaluate the metastatic ability of cancer cells. In addition, the assay can also be used to assess the effect of certain ...
[摘要] 细胞粘附，细胞与细胞外基质（ECM），其他细胞或特定表面的结合对于细胞的生长和存活以及其与其它细胞的连通是必需的。细胞粘附的过程涉及一系列生物事件，例如细胞骨架的三维重组，细胞中的生化反应和细胞表面上分子的变化。癌细胞，特别是高度转移的类型，被认为具有增强的粘附能力，通常促进细胞迁移到新的位置以在体内建立新的肿瘤。因此，细胞粘附测定常用于评价癌细胞的转移能力。此外，该测定也可以用于评价某些治疗（例如，暴露于化学品）对细胞粘附能力的影响。本文描述了修饰的细胞粘附测定方案，用于研究细胞和细胞外物质之间的相互作用。
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| Scratch Wound Healing Assay
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Author:
Date:
2012-03-05
[Abstract] The scratch wound healing assay has been widely adapted and modified to study the effects of a variety of experimental conditions, for instance, gene knockdown or chemical exposure, on mammalian cell migration and proliferation. In a typical scratch wound healing assay, a “wound gap” in a cell monolayer is created by scratching, and the “healing” of this gap by cell migration and growth towards the center of the gap is monitored and often quantitated. Factors that alter the motility and/or growth of the cells can lead to increased or decreased rate of “healing” of the gap (Lampugnani, 1999). This assay is simple, inexpensive, and experimental conditions can be easily adjusted for different purposes. The assay can also be used for a high-throughput screen platform if an automated system ...
[摘要] 划伤伤口愈合测定法已经广泛适应和修改以研究多种实验条件例如基因敲低或化学暴露对哺乳动物细胞迁移和增殖的影响。 在典型的伤口创伤愈合测定中，通过刮擦产生细胞单层中的"伤口间隙"，并且监测并常常定量通过细胞迁移和朝向间隙中心的生长的该间隙的"愈合"。 改变细胞运动性和/或生长的因素可导致间隙"愈合"的增加或减少的速率（Lampugnani，1999）。 该测定简单，便宜，并且可以容易地针对不同目的调整实验条件。 如果使用自动化系统，该测定也可以用于高通量筛选平台（Yarrow和Perlman，2004）。
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| Transwell Cell Migration Assay Using Human Breast Epithelial Cancer Cell
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Author:
Date:
2012-02-20
[Abstract] Transwell migration assays have been widely used for studying the motility of different types of cells including metastatic cancer cells. The assay is also useful in screens for compounds that act as chemoattractants or inhibitors of chemotaxis for cells. The assay employs a permeable layer of support, usually a tissue-culture-treated microporous membrane, which is positioned between two compartments that mimic two different sets of microenvironments for cell survival/growth. Cells on one side of the membrane, when sensing chemoattractants placed on the other side of the compartment that diffuses through the membrane, can migrate through the pores in the membrane towards the source of the chemoattractants. Cells that migrate across the membrane can be quantified by fixing and counting. ...
[摘要] Transwell迁移测定已经广泛用于研究不同类型的细胞（包括转移性癌细胞）的运动性。该测定法还可用于筛选充当化学趋化剂或细胞趋化性抑制剂的化合物。该测定使用可渗透的支持层，通常是组织培养处理的微孔膜，其位于模拟用于细胞存活/生长的两种不同组的微环境的两个区室之间。膜的一侧上的细胞，当感测放置在通过膜扩散的隔室的另一侧上的化学引诱物时，可以通过膜中的孔向化学引诱物的来源迁移。穿过膜迁移的细胞可以通过固定和计数来定量。人乳腺上皮腺癌MD-231细胞生长相对较快并且是转移性的。在此使用MB-231细胞系来描述使用transwell装置的体外细胞迁移测定的程序。
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