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Wizard® SV Genomic DNA Purification System

Wizard ® SV基因组DNA纯化系统

Company: Promega
Catalog#: A2361
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Construction of Deletion-knockout Mutant Fowlpox Virus (FWPV)
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Date:
2014-05-20
[Abstract]  The construction of deletion-knockout poxviruses is a useful approach to determining the function of specific virus genes. This protocol is an adaptation of the transient dominant knockout selection protocol published by Falkner and Moss (1990) for use with vaccinia virus. The protocol makes use of the dominant selectable marker Escherichia coli guanine phosphoribosyltransferase (gpt) gene (Mulligan and Berg, 1981), under the control of an early/late poxvirus promoter. The deletion viruses that are produced no longer contain a selectable marker, which may be preferable for the production of vaccines. [摘要]  缺失敲除痘病毒的构建是确定特定病毒基因的功能的有用方法。 该方案是由Falkner和Moss(1990)公布的用于牛痘病毒的瞬时显性敲除选择方案的改编。 该方案利用在早期/晚期痘病毒启动子控制下的显性选择标记大肠杆菌鸟嘌呤磷酸核糖基转移酶(gpt)基因(Mulligan和Berg,1981)。 产生的缺失病毒不再含有可选择标记,其可优选用于疫苗的生产。

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