Paraplast Plus®
Company: Sigma-Aldrich
Catalog#: P3683
Product evaluation:
Imaging and Measurement of Nanomechanical Properties within Primary Xylem Cell Walls of Broadleaves
Author:
Date:
2014-12-20
[Abstract]  A technique of atomic force microscopy (AFM) called PeakForce quantitative nanomechanical mapping (PeakForce QNM) is an efficient tool for the quantitative mechanobiological imaging of fibrillar aggregate, human epidermal cell and woody plant cell wall topography (Sweers et al., 2011; Heu et al., 2012; Ďurkovič et al., 2012; Ďurkovič et al., 2013). Here, we describe a detailed protocol for the measurement of nanomechanical properties of primary xylem cell ...

Maize Kernels – Fixation in FAA, Embedding, Sectioning and Feulgen Staining
Author:
Date:
2013-08-05
[Abstract]   The protocol describes preparation of young developing maize kernels for microscopical analysis of nuclei in tissue sections. The fixative FAA (formaldehyde, acetic acid and ethanol) is suitable for preservation of nuclear morphology and allows for quantitative staining of DNA with Schiff reagent in Feulgen staining. The fixation and embedding protocol may be used also for various other histology staining procedures, but care must be taken as the cytoplasm usually shrinks a bit using this ...

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