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Glutaraldehyde solution

戊二醛溶液

Company: Sigma-Aldrich
Catalog#: G5882
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Nematode Epicuticle Visualisation by PeakForce Tapping Atomic Force Microscopy
Author:
Date:
2017-11-05
[Abstract]  The free-living soil nematode Caenorhabditis elegans has become an iconic experimental model animal in biology. This transparent animal can be easily imaged using optical microscopy to visualise its organs, tissues, single cells and subcellular events. The epicuticle of C. elegans nematodes has been studied at nanoscale using transmission and scanning (SEM) electron microscopies. As a result, imaging artefacts can appear due to embedding the worms into resins or coating the worms with a conductive gold layer. In addition, fixation and contrasting may also damage the cuticle. Conventional tapping mode atomic force microscopy (AFM) can be applied to image the cuticle of the dried nematodes in air, however this approach also suffers from imaging defects. Ideally, the ... [摘要]  自由生活的土壤线虫秀丽隐杆线虫已经成为生物学中一个标志性的实验动物模型。这种透明的动物可以很容易地使用光学显微镜成像,以可视化其器官,组织,单细胞和亚细胞事件。 C的角质层。已经使用透射和扫描(SEM)电子显微镜在纳米级研究了线虫线虫。结果,由于将蠕虫嵌入树脂或用导电金层涂覆蠕虫,可能出现成像伪像。另外,固定和对比也可能损伤角质层。传统的敲击模式原子力显微镜(AFM)可用于对干燥的线虫的角质层在空气中成像,然而这种方法也存在成像缺陷。理想情况下,线虫应该在类似自然环境的条件下成像。最近,我们报道了使用PeakForce攻丝AFM模式来成功实现可视化和数值表征。线虫角质层在空气和液体中均有表达(Fakhrullina et al。,2017)。我们成像的主要线虫表面结构和角质层的力学性能表征。该协议提供了对液体浸泡的AF的AFM成像的详细描述。线虫使用PeakForce攻丝原子力显微镜线虫。
【背景】自由生活和寄生虫的线虫由于其对农业和人类福祉的显着生物学效应而受到广泛的研究。显然,线虫中研究最多和最着名的物种是自由生活的土壤线虫秀丽隐杆线虫(Sterken等人,2015年)。这种蠕虫在许多研究中已被成功地用作多功能模式生物体(Fire等人,1998; Kenyon,2010; Swierczek等人,2011; O'Reilly等人,2014; ...

Scanning Electron Microscope (SEM) Imaging to Determine Inflorescence Initiation and Development in Olive
Author:
Date:
2017-10-05
[Abstract]  Here we present a protocol that describes how to image the structure of the olive axillary bud meristem with a scanning electron microscope (SEM) in order to characterize its identity and developmental stage. Briefly, the specimen is fixed with glutaraldehyde, saturated with ethanol, dried in a critical point dryer (CPD) system, dissected, coated with a conducting material and imaged with a scanning electron microscopy (SEM). [摘要]  在这里,我们提出一个协议,描述如何使用扫描电子显微镜(SEM)图像橄榄腋芽分生组织的结构,以表征其身份和发育阶段。 简言之,将样品用戊二醛固定,用乙醇饱和,在临界点干燥器(CPD)系统中干燥,解剖,用导电材料涂覆并用扫描电子显微镜(SEM)成像。
【背景】橄榄(Olea europaea)中开花诱导和花序开始的确切时间是争议的(Haberman等人,2017)。 在橄榄中,花序在冬季结束时从侧芽出现,春季开花。 我们制定了一个协议,通过在一年中的不同时间用SEM对橄榄芽中的分生组织进行成像,更好地表征橄榄花序开始的时间。 在这些SEM图像中,可以明确地确定分生组织结构,并且分生组织的定义水平可以高于以前研究中呈现的芽分生组织部分的图像。

Fluorescence in situ Localization of Gene Expression Using a lacZ Reporter in the Heterocyst-forming Cyanobacterium Anabaena variabilis
Author:
Date:
2017-01-05
[Abstract]  One of the most successful fluorescent proteins, used as a reporter of gene expression in many bacterial, plant and animals, is green fluorescent protein and its modified forms, which also function well in cyanobacteria. However, these fluorescent proteins do not allow rapid and economical quantitation of the reporter gene product, as does the popular reporter gene lacZ, encoding the enzyme β-galactosidase. We provide here a protocol for the in situ localization of β-galactosidase activity in cyanobacterial cells. This allows the same strain to be used for both a simple, quantitative, colorimetric assay with the substrate ortho-nitrophenyl-β-galactoside (ONPG) and for sensitive, fluorescence-based, cell-type localization of gene expression using ... [摘要]  在许多细菌,植物和动物中用作基因表达的报告基因的最成功的荧光蛋白之一是绿色荧光蛋白及其修饰形式,其在蓝细菌中也起良好作用。然而,与编码β-半乳糖苷酶的流行的报道基因lacZ一样,这些荧光蛋白不允许报道基因产物的快速和经济的定量。我们在这里提供了在蓝细菌细胞中原位β-半乳糖苷酶活性定位的方案。这允许将相同的菌株用于具有底物邻硝基苯基-β-半乳糖苷(ONPG)的简单,定量,比色测定,并且用于灵敏的,基于荧光的细胞型定位使用5-十二烷酰氨基荧光素二-β-D-吡喃半乳糖苷(C12-FDG)的基因表达。

背景 鱼腥藻变种是一种丝状蓝细菌,其区分称为异养细胞的特异性细胞,其特异性用于固氮(Kumar等人,2010; Maldener and Muro Pastor,2010)。由于在96孔中易于定量,酶,比色,β-半乳糖苷酶测定,我们使用大肠埃希氏菌的 lacZ 基因作为蓝细菌基因表达的转录报告基因(Griffith和Wolf,2002)和使用相同的菌株用于使用荧光底物5-十二烷基聚氨基荧光素二-β-D-吡喃半乳糖苷(C12-FDG)的原位定位基因表达的能力( Thiel等人,1995; ...

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