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Date:
2021-02-20
[Abstract] Current methods to obtain mesenchymal stem cells (MSCs) involve sampling, culturing, and expanding of primary MSCs from adipose, bone marrow, and umbilical cord tissues. However, the drawbacks are the limited numbers of total cells in MSC pools, and their decaying stemness during in vitro expansion. As an alternative resource, recent ceiling culture methods allow the generation of dedifferentiated fat cells (DFATs) from mature adipocytes. Nevertheless, this process of spontaneous dedifferentiation of mature adipocytes is laborious and time-consuming. This paper describes a modified protocol for in vitro dedifferentiation of adipocytes by employing an additional physical stimulation, which takes advantage of augmenting the stemness-related Wnt/β-catenin signaling. Specifically, this ...
[摘要] [摘要]目前的方法,以获得间充质干细胞(MSC)包括采样,培养,和扩大主要由脂肪,骨髓,和脐带组织的MSCs。然而,缺点是在总细胞在MSC池,和它们的衰减干性的数量有限在维生素- [R Ò扩张。作为替代资源,最近的天花板培养方法允许从成熟的脂肪细胞中生成去分化的脂肪细胞(DFAT)。然而,这种成熟脂肪细胞自发去分化的过程既费力又费时。本文描述了一种用于经修改协议在体外通过采用附加的物理刺激,其中脂肪细胞去分化TA KES扩充所述干性相关的优点的Wnt /β-catenin信号。具体来说,该协议利用含聚乙二醇(PEG)的高渗介质引入细胞外物理刺激以获得更高的效率,并引入更简单的脂肪细胞去分化程序。
[背景]脂肪组织由于其丰度大且侵袭性相对较低,因此是间充质干细胞(MSC)最具吸引力的来源之一(Shen等,2011 ;González-Cruz等,2012; Konno等人,2013)。脂肪来源的MSC,即从皮下脂肪组织的基质血管级分中分离,已被证实同时显示多谱系潜能的体外和体内(Anghileri等人,2008;冈萨雷斯。等人,2009;冈萨雷斯-雷伊等等人,2010; Jumabay等人,2010; Mao等人,2017和2019 ;Darnell等人,2018 ...
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