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PierceTM BCA Protein Assay Kit

Pierce TM BCA蛋白测定试剂盒

Company: Thermo Fisher Scientific
Catalog#: 23225
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Superoxide Dismutase (SOD) and Catalase (CAT) Activity Assay Protocols for Caenorhabditis elegans
Author:
Date:
2017-08-20
[Abstract]  Assays for superoxide dismutase (SOD) and catalase (CAT) activities are widely employed to indicate antioxidant responses underlying the toxic effects of test chemicals. Yet, earlier studies mainly described the procedures as performed according to manufacturer’s instructions without modifications that are specific to any organisms. The present protocol describes the steps in analyzing the superoxide dismutase (SOD) and catalase (CAT) activities in C. elegans, which is a model organism that can be used to study effects of pharmaceutical compounds and environmental pollutants. The main steps include: (1) sample preparation; (2) total protein assay; (3) SOD activity assay; (4) CAT activity assay; and (5) medium list and formula, and also data analysis and performance notes. [摘要]  超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性的测定被广泛用于表明测试化学品的毒性作用的抗氧化反应。 然而,早期的研究主要描述了根据制造商的说明进行的程序,而无需对任何生物体特异的修改。 本方案描述了分析线虫中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性的步骤,它是可用于研究药物化合物和环境污染物的影响的模型生物。 主要步骤包括:(1)样品制备; (2)总蛋白测定; (3)SOD活性测定; (4)CAT活性测定; 和(5)中等名单和公式,以及数据分析和绩效说明。
【背景】生物标志物是对化学,药剂或治疗干预的响应而检查生物和病原过程至关重要的。生物体内的各种生物过程导致引起氧化应激的活性氧(ROS)。为了应对这种氧化应激,生物体可以部署超氧化物歧化酶(SOD)和过氧化氢酶(CAT)以清除ROS,以保护细胞的稳态(Balaban等,2005)。一方面,各种化学物质(污染物)可以阻止这种抗氧化反应,并扰乱包括人类在内的生物体的健康。另一方面,许多药物旨在加强抗氧化反应以改善健康。因此,SOD和CAT的活动对于反映化学品或/和药物的潜在影响非常重要。
   秀丽隐杆线虫(秀丽隐杆线虫)是一种用于研究药物化合物(Dengg和van Meel,2004; Carretero等,2017)和环境污染物(Yu et ...

Assays for the Detection of Rubber Oxygenase Activities
Author:
Date:
2017-03-20
[Abstract]  Microbial biodegradation of rubber relies on extracellular rubber oxygenases that catalyze the oxidative cleavage of the double bond of the polyisoprene backbone into oligo-isoprenoids. This protocol describes the determination of rubber oxygenase activities by an online measurement of molecular oxygen consumption via a non-invasive fluorescence-based assay. The produced oligo-isoprenoid cleavage products with terminal keto- and aldehyde-groups are identified qualitatively and quantitatively by HPLC. Our method allows for the characterization of homologue rubber oxygenases, and can likely be adapted to assay other oxygenases consuming dioxygen. Here we describe the determination of rubber oxygenase activities at the examples of the so far two known types of rubber oxygenases, ... [摘要]  橡胶的微生物生物降解依赖于细胞外橡胶加氧酶,其催化聚异戊二烯骨架的双键氧化裂解成低聚异戊二烯。该方案描述了通过基于非侵入性荧光的测定在线测量分子氧消耗来测定橡胶加氧酶活性。通过HPLC定性和定量鉴定产生具有末端酮基和醛基的寡聚异戊二烯切割产物。我们的方法允许表征同系物橡胶加氧酶,并且可能适用于测定消耗双氧的其他加氧酶。在这里,我们描述了迄今为止两种已知类型的橡胶加氧酶,即橡胶加氧酶A(RoxA)和胶乳清除蛋白(Lcp)的实例的橡胶加氧酶活性的测定。

背景 ...

An HPLC-based Method to Quantify Coronatine Production by Bacteria
Author:
Date:
2017-03-05
[Abstract]  Coronatine is a polyketide phytotoxin produced by several pathovars of the plant pathogenic bacterium Pseudomonas syringae. It is one of the most important virulence factors determining the success of bacterial pathogenesis in the plant at both epiphytic and endophytic stages of the disease cycle. This protocol describes an optimized procedure to culture bacterial cells for coronatine production and to quantify the amount of coronatine secreted in the culture medium using an HPLC-based method. [摘要]  冠心病是由植物病原菌的几种病原体产生的聚酮化合物植物毒素。它是在疾病周期的附生和内生期阶段确定植物细菌发病机制成功的最重要的毒力因素之一。该方案描述了用于培养用于冠状病毒产生的细菌细胞的优化方法,并且使用基于HPLC的方法定量培养基中分泌的冠状动脉的量。

背景 Coronatine(COR)是有效的细菌植物毒素,是植物激素茉莉酮酸-L异亮氨酸(JA-Ile)的分子模拟物。因此,COR激活茉莉酸(JA)信号传导,诱导JA响应基因,并拮抗免疫信号水杨酸的作用。 COR由两种成分组成:冠心病(CFA)和冠状氨酸(CMA)。编码CMA和CFA生物合成的基因在细菌中不是组成型表达的。相反,当细菌在诱导培养基中生长时,这些基因在植物叶片表面或植物体内或/或体外诱导(Palmer和Bender,1993; Panchal等等,2016)。本文介绍了从Panchal等人改编的方法。 (2016),以确定细菌产生冠心病的能力,可用作毒性指征。

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