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Author:
Date:
2014-03-20
[Abstract] Fitness is defined as the overall replicative ability. Testing whether a mutation (or combination of mutations) has an effect on fitness often relays on determining virus production as a surrogate measurement. However, viruses do not usually replicate in a void, and evolutionary speaking, it is key to determine replicative ability compared to other viruses, e.g. the relative fitness. John Holland developed a method for vesicular stomatitis virus based on the use of a neutral genetic marker that allows to distinguish two competitors and to measure accurately the relative ratio between the two during competition (Holland et al., 1991). The marker is a mutation in the external G glycoprotein that has no effect on the virus other than conferring resistance to a monoclonal ...
[摘要] 健身被定义为整体的复制能力。测试突变（或突变的组合）是否对适应性有影响经常决定作为替代测量的病毒生产。然而，病毒通常不在空白中复制，并且在进化论上，确定与其他病毒相比的复制能力是关键，例如相对适合度。 John Holland开发了一种基于使用中性遗传标记物的水疱性口炎病毒的方法，所述中性遗传标记物允许区分两个竞争者并且准确地测量竞争期间两者之间的相对比率（Holland等人，1991 ）。标记是外部G糖蛋白中的突变，其对赋予对单克隆抗体I1的抗性以外的病毒没有影响。为了测量适应度，将标记的测试菌株与参考未标记的菌株混合，并使混合物感染细胞单层。通过在I1抗体存在和不存在下的噬斑测定来测量竞争前后的比率，比率的变化给出适合度值。
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