|
Author:
Date:
2013-12-05
[Abstract] Outer membrane vesicles (OMVs) are spherical bilayered phospholipids of 20-200 nm in size produced from all Gram-negative bacteria and Gram-positive bacteria investigated to date. OMVs, which resemble the outer membrane and periplasm in composition, are proinflammatory and immunogenic facsimiles, and therefore could activate both innate and adaptive immunity. Here, we describe the OMVs immunization protocol and bacteria challenge protocol to induce bacterial sepsis in mice.
[摘要] 外膜囊泡（OMV）是从所有革兰氏阴性细菌和革兰氏阳性细菌产生的20-200nm的球形双层磷脂，迄今为止研究。 在组成上类似于外膜和周质的OMV是促炎和免疫原性传真，因此可以激活先天免疫和适应性免疫。 在这里，我们描述OMVs免疫协议和细菌攻击协议，以诱导小鼠的细菌性败血症。
|