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"The Big Easy" EasySepTM Magnet

“大容易”EasySep TM磁铁
Company: STEMCELL Technologies
Catalog#: 18001
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Adoptive Transfer of Memory B Cells
Author:
Date:
2015-08-20
[Abstract]  The adoptive transfer of antigen-specific B cells into mice that cannot recognize that specific antigen has two main advantages. The first is determining exactly when the B cells were transferred and exposed to antigen. The second is that all B cells that can bind that antigen are the ones that were transferred; no new antigen-specific B cells will emerge from the bone marrow. Thus all B cells that were exposed to the antigen and still alive after at least 4 weeks (8 weeks or more is ideal), are memory B cells.

Splenic B cells from B1-8 mice were prepared with an EasySep Mouse B Cell Enrichment Kit according to the manufacturer’s protocol. Single-cell suspensions were transferred intravenously into tail veins of recipient mice. Approximately 1 million NP+ B cells were ... [摘要]  将抗原特异性B细胞过继转移到不能识别该特异性抗原的小鼠中具有两个主要优点。 第一种是确切地确定B细胞何时转移并暴露于抗原。 第二是可以结合该抗原的所有B细胞是转移的; 从骨髓中不会出现新的抗原特异性B细胞。 因此，暴露于抗原并在至少4周（8周或更长时间是理想的）后仍存活的所有B细胞是记忆B细胞。
根据制造商的方案用EasySep小鼠B细胞富集试剂盒制备来自B1-8小鼠的脾B细胞。 将单细胞悬浮液静脉内转移到受体小鼠的尾静脉中。 每只小鼠转移约100万个NP + B细胞。 转移后约12-24小时，用在明矾中沉淀的50μgNP-CGG腹膜内免疫小鼠。

Isolation of CD34+ Cells from Human Fetal Liver and Cord Blood
Author:
Date:
2013-12-05
[Abstract]  CD34 is a glycosylated cell surface protein and represents a well-known marker for primitive progenitor cells in various organs, especially cord blood, bone marrow and fetal liver. CD34+ progenitor cells are suitable for a series of studies, e.g. cell differentiation, transplantation as well as construction of humanized mouse models. Here, we describe a method to isolate CD34+ cells from the human cord blood and fetal liver.
[摘要]  CD34是糖基化的细胞表面蛋白，并且代表了在各种器官，特别是脐带血，骨髓和胎儿肝脏中的原始祖细胞的公知标记。 CD34 +祖细胞适合于一系列研究，例如细胞分化，移植以及人源化小鼠模型的构建。 在这里，我们描述了一种从人脐带血和胎儿肝脏中分离CD34 - sup + +细胞的方法。

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