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Thermolysin from Geobacillus stearothermophilus

嗜热脂肪地芽孢杆菌的嗜热菌蛋白酶

Company: Sigma-Aldrich
Catalog#: P1512
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Preparation of Primary Neurons from Rat Median Preoptic Nucleus (MnPO)
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2013-12-05
[Abstract]  Studying cell physiology is really important to understand their function and to determine action mechanisms taking place in these cells. Using brain slices can be sometime difficult to directly access to cells like neurons. Even if it more steps are needed to get cultured cells, this type of preparation allow a better access to neurons and provide a good way to study their basal properties. Here we describe a protocol of a short term primary neurons culture from MnPO, allowing to kept neurons in good condition to perform electrophysiological recordings. [摘要]  研究细胞生理学对于了解其功能和确定在这些细胞中发生的作用机制是非常重要的。 使用脑切片可能有时难以直接访问像神经元这样的细胞。 即使需要更多的步骤来培养细胞,这种类型的制剂可以更好地接近神经元,并提供研究其基础性质的好方法。 在这里,我们描述了一种来自MnPO的短期原代神经元培养的方案,允许神经元保持良好的状态进行电生理记录。

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