| In vitro Differentiation of Murine Innate Lymphoid Cells from Common Lymphoid Progenitor Cells
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Author:
Date:
2016-03-20
[Abstract] Subtypes of innate lymphoid cells (ILC), defined based on their cytokine secretion profiles and transcription factor expression, are important for host protection from pathogens and maintaining tissue homeostasis. ILCs develop from common lymphoid progenitors (CLP) in the bone marrow. Using the methods described here, we have previously shown that loss of the transcriptional regulator TOX (Thymocyte-selection associated HMG-box protein) leads to specific changes in ILC development and differentiation. Here, we describe how to obtain ILCs from in vivo isolated CLP grown in vitro.
[摘要] 先天淋巴细胞（ILC）的亚型，基于其细胞因子分泌谱和转录因子表达来定义，对于宿主保护免于病原体和维持组织内稳态是重要的。 ILC从骨髓中的普通淋巴祖细胞（CLP）发展而来。 使用本文所述的方法，我们以前显示转录调节器TOX（胸腺细胞选择相关的HMG框蛋白）的损失导致ILC发展和分化的具体变化。 在这里，我们描述了如何从体内分离的CLP获得ILC。
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| Adherent-invasive Escherichia coli Biofilm Formation Assays
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Author:
Date:
2013-12-05
[Abstract] Patients with Crohn’s disease are abnormally colonized by adherent-invasive Escherichia coli (AIEC) bacteria (Chassaing and Darfeuille-Michaud, 2011). These bacteria are able to adhere to and invade intestinal epithelial cells (IEC), to replicate within macrophages, and were recently described to be able to form biofilms (Martinez-Medina et al., 2009; Chassaing and Darfeuille-Michaud, 2013). The reference strain of adherent-invasive E. coli is the strain LF82, associated with ileal Crohn’s disease (Darfeuille-Michaud et al., 1998).
This protocol described basic steps of a biofilm formation assay on I) non-cell-treated polystyrene microtiter plates and on II) paraformaldehyde-fixed I-407 IEC monolayers.
[摘要] 克罗恩病患者被粘附侵入性大肠杆菌（AIEC）细菌异常定植（Chassaing和Darfeuille-Michaud，2011）。 这些细菌能够粘附和侵入肠上皮细胞（IEC），在巨噬细胞内复制，并且最近被描述为能够形成生物膜（Martinez-Medina等人，2009; Chassaing和 Darfeuille-Michaud，2013）。 粘附侵入性E的参考应变。 大肠杆菌是与回肠克罗恩病相关的菌株LF82（Darfeuille-Michaud等人，1998）。
该方案描述了生物膜形成测定的基本步骤：I） 非细胞处理的聚苯乙烯微量滴定板和II）多聚甲醛固定的I-407 IEC单层。
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