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Author:
Date:
2017-07-05
[Abstract] The ubiquitously expressed bromodomain-containing protein 4 (BRD4) is an epigenetic reader, which recruits transcriptional regulatory complexes to acetylated chromatin. Because of its role in enhancing proliferation, BRD4 has become a therapeutic target in oncology, as the inhibition of this protein leads to the reduction of the growth of many tumours. Even though BRD4 is more and more studied, its mechanism of action has not been fully described yet. Therefore, we aimed at generating a cellular reporter system to monitor BRD4 inhibition. Such reporter can be potentially used in high throughput chemical and genetic screenings, in order to uncover new possible BRD4 functional pathways. The deeper understanding of the mechanism of action of BRD4 activity will certainly help in developing ...
[摘要] 普遍表达的含溴结构域的蛋白质4（BRD4）是表观遗传学读者，其将转录调节复合物募集到乙酰化染色质。 由于其在增强增殖中的作用，BRD4已经成为肿瘤学的治疗靶点，因为抑制这种蛋白质导致许多肿瘤的生长减少。 即使BRD4越来越多的研究，其行动机制尚未得到充分的描述。 因此，我们旨在产生细胞报告系统来监测BRD4抑制。 这种记录可以潜在地用于高通量化学和遗传筛选，以揭示新的可能的BRD4功能途径。 对BRD4活动作用机制的深入了解肯定有助于为所谓的BRD4依赖型癌症开发新的治疗策略。
【背景】表观遗传学领域的研究最近突出了BRD4在癌症进展中的中心作用。 BRD4是BET（溴结构域和终末外结构域）家族（Dey等人，2003; Filippakopoulos等人，2012; Wang）的乙酰赖氨酸阅读器， et al。，2012）能够结合启动子和增强子区域上的乙酰化组蛋白（Dey等人，2003; Filippakopoulos等人，2012; Nagarajan等人，，2014）。这种表观遗传学读者的作用机制在于通过招募几种转录因子，辅因子和RNA聚合酶II（RNApol II）来激活基因启动子和增强子，其导致调节，大部分增强某些靶基因的转录。已经描述了BRD4组蛋白模块发挥调控细胞周期进程的关键作用（Dey等人，2003; Wu和Chiang，2007; Yang等人， ...
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Author:
Date:
2012-03-05
[Abstract] Cell adhesion, the binding of a cell to the extracellular matrix (ECM), other cells, or a specific surface, is essential for the growth and survival of the cell and also its communication with other cells. The process of cell adhesion involves a range of biological events such as three-dimensional re-organization of the cytoskeleton, biochemical reactions in the cell, and changes in molecules on the surface of the cell. Cancer cells, especially the highly metastatic types, are believed to have enhanced adhesion ability that often facilitates the migration of the cells to a new site to establish new tumors in the body. Cell adhesion assay is therefore often used to evaluate the metastatic ability of cancer cells. In addition, the assay can also be used to assess the effect of certain ...
[摘要] 细胞粘附，细胞与细胞外基质（ECM），其他细胞或特定表面的结合对于细胞的生长和存活以及其与其它细胞的连通是必需的。细胞粘附的过程涉及一系列生物事件，例如细胞骨架的三维重组，细胞中的生化反应和细胞表面上分子的变化。癌细胞，特别是高度转移的类型，被认为具有增强的粘附能力，通常促进细胞迁移到新的位置以在体内建立新的肿瘤。因此，细胞粘附测定常用于评价癌细胞的转移能力。此外，该测定也可以用于评价某些治疗（例如，暴露于化学品）对细胞粘附能力的影响。本文描述了修饰的细胞粘附测定方案，用于研究细胞和细胞外物质之间的相互作用。
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