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Cell Culture Grade water

细胞培养级水

Company: Cytiva
Catalog#: SH30529.03
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Harvest and Culture of Mouse Peritoneal Macrophages
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2013-11-20
[Abstract]  Peritoneal macrophages are used as primary macrophages in lots of studies, mainly because they are easy to obtain. Injection of thioglycollate broth i.p. induces inflammatory responses and elicits large numbers of macrophages. This protocol can be used for harvesting resident or thioglycollate-elicited peritoneal cells. Peritoneal macrophages are non-adherent in situ and when they are cultured in dishes, they become adherent so that macrophages may be separated from other types of cells in peritoneal cavity. [摘要]  腹膜巨噬细胞在许多研究中用作初级巨噬细胞,主要是因为它们容易获得。 注射巯基乙酸盐肉汤i.p. 诱导炎症反应并引发大量的巨噬细胞。 该方案可用于收获驻留的或巯基乙酸盐诱发的腹膜细胞。 腹膜巨噬细胞是原位非粘附的,并且当它们在培养皿中培养时,它们变得粘附,使得巨噬细胞可以与腹膜腔中的其它类型的细胞分离。

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