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DQTM Ovalbumin

DQ TM卵清蛋白

Company: Thermo Fisher Scientific
Catalog#: D12053
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Assessment of Human Dendritic Cell Antigen Uptake by Flow Cytometry
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2013-11-20
[Abstract]  Antigen uptake by dendritic cells is the first key step towards induction of antigen-specific T-cell responses. This flow cytometry-based protocol describes the analysis of dendritic cell uptake of soluble antigens through two different mechanisms: non-specific macropinocytosis (using Lucifer Yelloy CH), and receptor-mediated endocytosis (using DQTM Ovalbumin). The protocol is generated based on data presented in Olivar et al. (2013). [摘要]  树突状细胞的抗原摄取是诱导抗原特异性T细胞应答的第一个关键步骤。 这种基于流式细胞术的方案描述了通过两种不同的机制分析树突状细胞摄取可溶性抗原:非特异性大细胞增多症(使用Lucifer Yelloy CH)和受体介导的内吞作用(使用DQ TM 卵清蛋白) 。 该方案是基于Olivar等人(2013)中提供的数据产生的。

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