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Author:
Date:
2013-11-05
[Abstract] Direct visualization of the organization and dynamics of the actin cytoskeleton in rice cells is essential to understand its roles in regulating rice growth and development. Visualization of actin dynamics in protoplasts transformed with actin probe is a relatively quick and simple strategy, compared to the strategy of generating stable transgenic rice plants that harbor actin probe, which is time-consuming. Here is a protocol described in details regarding transforming rice protoplasts as well as visualizing and quantifying actin dynamics in rice protoplasts, which is based on the method previously reported (Shi et al., 2013).
[摘要] 直接可视化的肌动蛋白细胞骨架在水稻细胞的组织和动态是必不可少的理解其在调节水稻生长和发展中的作用。 与产生含有肌动蛋白探针的稳定转基因水稻植物的策略相比，用肌动蛋白探针转化的原生质体中肌动蛋白动力学的可视化是相对快速和简单的策略，这是耗时的。 这里是详细描述关于转化水稻原生质体以及可视化和量化水稻原生质体中的肌动蛋白动力学的方案，其基于先前报道的方法（Shi等人，2013）。
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