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Author:
Date:
2016-01-05
[Abstract] Cell migration is a highly complex and dynamic biological process, essential in several physiological phenomena and pathologies including cancer dissemination and metastasis formation. Thus understanding single cell migration is highly relevant and requires a suitable image-based assay. Depending on the speed of the moving cells, one may require fast time-lapse microscopy, which is not always suitable for high-throughput screening. To overcome this, a quantitative and fixed single cell migration assay was developed based on the PhagoKinetic Tracks (PKT) procedure. Briefly, cells are seeded on top of a monolayer of carboxylated latex beads, and as cells migrate, they phagocytose these beads and leave behind a migratory track. These bead-free migratory tracks can be visualized using a ...
[摘要] 细胞迁移是高度复杂和动态的生物过程，在几种生理现象和病理包括癌症传播和转移形成中是必需的。因此，理解单细胞迁移是高度相关的，并需要合适的基于图像的测定。根据移动细胞的速度，可能需要快速时间延迟显微术，这不总是适合于高通量筛选。为了克服这一点，基于Phago运动轨迹（PKT）程序开发了定量和固定的单细胞迁移测定。简言之，将细胞接种在单层羧化的乳胶珠的顶部，并且当细胞迁移时，它们吞噬这些珠并留下迁移轨迹。这些无珠移动轨迹可以使用标准明场显微镜可视化，并分析单细胞迁移的多参数定量评估（Naffar-Abu-Amara等人，2008）。
在这里，我们描述了PKT测定的详细和优化的方案，适用于RNAi和药物筛选（van Roosmalen等人，2015）。这个协议允许用户在单细胞水平研究迁移行为，没有快速和活成像显微镜。
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Author:
Date:
2013-11-05
[Abstract] This is a rapid protocol to test the effects of drugs treatment on bovine cell replication using Ki67 staining. Ki67 is associated with cell proliferation and is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0).
[摘要] 这是一个快速协议，以测试药物治疗对牛细胞复制使用Ki67染色的影响。 Ki67与细胞增殖相关，并且在细胞周期（G1，S，G2和有丝分裂）的所有活性期期间存在，但在静息细胞（G0）中不存在。
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