| Isolation of Persister Cells from Biofilm and Planktonic Populations of Pseudomonas aeruginosa
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Author:
Date:
2015-09-20
[Abstract] Persister cells are a stochastically produced sub-population of non-growing bacterial cells. Recently these cells have been more widely studied due to the recognition that they are tolerant to antimicrobials and thus, play a major role in the resilience of bacterial populations to antimicrobials, particularly in chronic biofilm infections. The following protocol describes the isolation/selection of persister cell sub-populations of Pseudomonas aeruginosa present in biofilms (sessile) and planktonic populations (free-living).
[摘要] 滞留细胞是非生长细菌细胞的随机产生的亚群。 最近，这些细胞已被更广泛地研究，因为它们耐受抗微生物剂并因此在细菌群体对抗微生物剂，特别是慢性生物膜感染的弹性中起主要作用。 以下方案描述了存在于生物膜（无菌）和浮游群体（自由生活）中的铜绿假单胞菌的持续细胞亚群的分离/选择。
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| Quantification of Respiratory Activity in Biofilms
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Author:
Date:
2015-09-20
[Abstract] Bacteria live mostly as biofilms, not as planktonic cell populations. Bacterial cells living as biofilms are known to be in different physiological status. Persister cells are one of such physiological conditions and they are recognized as to be a stochastically produced sub-population of non-growing bacterial cells. The following protocol describes a method to determine the respiratory activity of cells within biofilms.
[摘要] 细菌主要作为生物膜，而不是作为浮游细胞群。 作为生物膜存在的细菌细胞已知处于不同的生理状态。 持续细胞是这样的生理条件之一，并且它们被认为是非生长细菌细胞的随机产生的亚群。 以下方案描述了确定生物膜内细胞的呼吸活性的方法。
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| H2O2 Kill Assays of Biofilm Bacteria
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Author:
Date:
2013-11-05
[Abstract] Ubiquitous in nature and often surface associated, biofilms cause numerous chronic human infections. Biofilms are structured multicellular bacterial communities where cells are entrapped in a polymer matrix. Bacteria growing as biofilms are characterized by marked tolerance to many biocides, including oxidants such as hydrogen peroxide. Hydrogen peroxide is both produced by host phagocytic cells, and used as an antimicrobial compound. Understanding biofilm tolerance to hydrogen peroxide is therefore relevant to the persistence of Pseudomonas aeruginosa in human infections (such as chronic Pseudomonas aeruginosa infections in cystic fibrosis airways) as well as in environmental settings (such as water pipes).
This protocol was developed to determine the ...
[摘要] 无处不在的自然和经常表面相关，生物膜导致许多慢性人类感染。生物膜是结构化的多细胞细菌群落，其中细胞被包埋在聚合物基质中。作为生物膜生长的细菌的特征在于对许多杀生物剂包括氧化剂如过氧化氢的显着耐受性。过氧化氢由宿主吞噬细胞产生，并用作抗微生物化合物。因此，了解对过氧化氢的生物膜耐受性与人类感染（例如囊性纤维化气道中的慢性绿脓假单胞菌感染）以及环境设置中的铜绿假单胞菌的持久性有关（如水管）。
该方案用于确定绿脓杆菌生物膜对过氧化氢（H 2 O 2 O 2）杀伤的耐受性。细菌作为菌落生物膜在聚碳酸酯膜上生长，如先前在Walters等人2003中所述。该方案可以适用于其它细菌，在H 2亚型中具有适当的变化， O 2浓度，因为不同的细菌物种可能比H 2 O 2比对绿脓杆菌更敏感或更不敏感。
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