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Author:
Date:
2013-10-20
[Abstract] Trehalose is a nonreducing disaccharide. It is a common sugar in bacteria, fungi and yeast, where it functions as a carbon source and stress protectant. In contrast, plants, although encoding large trehalose biosynthesis gene families, contain only small amounts of trehalose. The intermediate compound of trehalose, trehalose-6-phosphate (T6P), is a signaling molecule in plants, regulating metabolism, growth, and development. Most plants contain only a single trehalase, the enzyme that specifically hydrolyzes trehalose into two glucose molecules. High trehalase activity has been suggested to be part of the defense mechanism in plants hosting mycorrhizal fungi, rhizobia, and the plant pathogen Plasmodiophora brassica. Recently, it was shown in Arabidopsis thaliana that ...
[摘要] 海藻糖是非还原二糖。它是细菌，真菌和酵母中的常见糖，其在其中用作碳源和应激保护剂。相反，植物虽然编码大的海藻糖生物合成基因家族，但仅含有少量的海藻糖。海藻糖，海藻糖-6-磷酸（T6P）的中间体化合物是植物中的信号分子，调节代谢，生长和发育。大多数植物仅含有单一海藻糖酶，该酶特异性地将海藻糖水解成两个葡萄糖分子。已经提出高海藻糖酶活性是植物接种菌根真菌，根瘤菌和植物病原体禾本科植物的防御机制的一部分。最近，在拟南芥中显示高海藻糖酶活性与干旱胁迫耐受性的增加相关，并且海藻糖酶在气孔调节中实现重要作用。在这里，我们描述了测定拟南芥组织中的海藻糖酶活性的方案，优化用于96孔板。透析的蛋白质提取物将与海藻糖一起温育，随后使用葡萄糖氧化酶 - 过氧化物酶定量释放的葡萄糖。
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