{{'Search' | translate}}
 

Non-invasive Micro-test System

非侵入性微测试系统

Company: YoungerUSA
Catalog#: NMT100
Bio-protocol()
Company-protocol()
Other protocol()

Measurement of Extracellular Ca2+ Influx and Intracellular H+ Efflux in Response to Glycerol and PEG6000 Treatments
Author:
Date:
2013-09-20
[Abstract]  The characteristics of Ca2+ and H+ fluxes may reflect the activities of aquaporins, as the up-regulation of aquaporin activities is directly associated with the decrease in cytoplasmic H+ concentration and increase in cytoplasmic Ca2+ concentration. The higher aquaporin activities can protect cells against osmotic stresses by altering water flow into and out of the cells. In order to confirm the contribution of aquaporins to the cell tolerance to different osmotic stresses, net Ca2+ and H+ fluxes are measured using the noninvasive micro-test technique (NMT). NMT provides the real-time in situ detection of net ion transport across membranes. Here, we describe the protocol of in situ detection of net Ca2+ ... [摘要]  Ca 2+和H sup +通量的特征可反映水通道蛋白的活性,因为水通道蛋白活性的上调与细胞质H的减少直接相关> + 浓度和细胞质Ca 2+浓度的增加。较高的水通道蛋白活性可以通过改变进入和离开细胞的水流来保护细胞免受渗透压力。为了证实水通道蛋白对细胞对不同渗透胁迫的耐受性,使用非侵入性微测试技术(NMT)测量净Ca 2+和H + +通量, )。 NMT提供了跨膜的净离子迁移的实时原位检测。在这里,我们描述了原位检测穿过转化的巴斯德毕赤氏酵母的净Ca 2+和 + 通量的方案。 >细胞响应于甘油和聚乙二醇6000(PEG6000)处理。将转化的酵母细胞加载到在聚-L-赖氨酸溶液(0.1%w/v水溶液)中预处理的盖片上。细胞固定后,微电极位于单层细胞群上方。在由计算机操纵的两个偏移点处测量微伏电压差。使用ASET 2.0和iFluxes 1.0软件,微伏电压差可以转换为离子通量。该方法有望促进NMT在微生物学中的应用。我们非常感谢Younger USA(徐越北京)NMT服务中心对稿件的批判性阅读。

Comments