RNA PowerSoil^® Total RNA Isolation Kit

RNA PowerSoil ^® Total RNA Isolation Kit

Company: MO BIO Laboratories

Catalog#: 12866-25

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Purification of Bacterial RNA from Infected Macrophages

Lior Lobel, Nadejda Sigal, Anna Pasechnek and Anat A. Herskovits

Published online: 2015-11-20

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Isolating RNA from the Soil

Author:

Jacqueline M Chaparro and Jorge M Vivanco,

Date:

2013-09-20

[Abstract] Next generation sequencing has allowed for the analysis and ability to identify the microbial communities present in the environment. While DNA extraction from environments (such as soil) have provided a wealth of knowledge regarding microbial communities there are drawbacks that one encounters when using DNA as opposed to RNA. RNA allows for the determination of the identity of the microbes that are active and present at a particular time point and thus gives a clear picture of what these microbes are actually doing at a specific point in time and under a set of conditions. Extracting RNA from soil is challenging due to the inherent inhibitors present in the soil such as humic acids. Here we describe modifications to the MoBio RNA PowerSoil^TM total RNA isolation kit to ... [摘要] 下一代测序允许分析和鉴定存在于环境中的微生物群落的能力。虽然从环境（例如土壤）中提取DNA提供了关于微生物群落的丰富知识，但是当使用DNA而不是RNA时会遇到缺点。 RNA允许测定活性并存在于特定时间点的微生物的身份，并因此给出这些微生物实际上在特定时间点和在一组条件下进行的清楚图片。从土壤中提取RNA是具有挑战性的，因为存在于土壤中的固有抑制剂如腐殖酸。在这里我们描述修改的MoBio RNA PowerSoil ^TM总RNA分离试剂盒从土壤中重复提取总RNA。

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RNA PowerSoil® Total RNA Isolation Kit

RNA PowerSoil ® Total RNA Isolation Kit

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RNA PowerSoil^® Total RNA Isolation Kit

RNA PowerSoil ^® Total RNA Isolation Kit