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Author:
Date:
2013-09-05
[Abstract] A hallmark of cancer-associated inflammation is the infiltration of leukocytes into tumors, which is believed to be recruited by chemokines. Some infiltrating leukocytes such as macrophages often promote tumor growth by producing growth-inducing and angiogenic factors. Here, we described a method of isolating tumor-infiltrating leukocytes with Percoll density gradient, because Percoll possesses a low viscosity, a low osmolarity and no toxicity to cells. Different leukocyte populations are isolated based on their density and collected at the interface between 40% and 80% discontinuous Percoll gradient.
[摘要] 癌症相关炎症的标志是白细胞浸润到肿瘤中，据信这是由趋化因子募集的。 一些浸润性白细胞如巨噬细胞通常通过产生生长诱导和血管生成因子来促进肿瘤生长。 在这里，我们描述了一种用Percoll密度梯度分离肿瘤浸润白细胞的方法，因为Percoll具有低粘度，低渗透压和对细胞无毒性。 基于它们的密度分离不同的白细胞群体，并在40％和80％不连续的Percoll梯度之间的界面处收集。
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