| Colon Cancer-associated Fibroblast Establishment and Culture Growth
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Author:
Date:
2016-04-05
[Abstract] Cancer-associated fibroblasts (CAFs) are one of the major players in tumor-stroma crosstalk. Findings in experimental studies suggest important roles for CAFs in regulation of tumor growth, metastasis and drug response (Hanahan and Coussens, 2012). Furthermore, their clinical relevance is supported by new findings from tumor analyses, demonstrating the prognostic and response-predictive significance of CAF-derived markers or gene signatures (Berdiel-hacer et al., 2014; Finak et al., 2008; Navab et al., 2011; Paulsson and Micke, 2014). CAFs are a heterogeneous pool of cell subsets with distinct functions which needs to be better defined by their marker expressions. The development of a methodology for the establishment of fibroblast primary cultures derived from ...
[摘要] 癌症相关成纤维细胞（CAF）是肿瘤 - 基质串扰的主要参与者之一。实验研究的结果表明CAFs在肿瘤生长，转移和药物反应的调节中的重要作用（Hanahan和Coussens，2012）。此外，它们的临床相关性得到来自肿瘤分析的新发现的支持，表明CAF衍生的标记或基因特征的预后和应答预测显着性（Berdiel-hacer等人，2014; Finak 等人，2008; Navab等人，2011; Paulsson和Micke，2014）。 CAF是具有不同功能的细胞亚群的异质池，其需要由它们的标记表达更好地定义。开发用于建立源自人结肠肿瘤的成纤维细胞原代培养物的方法允许我们表征其功能和分子性质（Herrera等人，2013）。此外，CAF影响肿瘤生长和转移的不同分子机制仍有待澄清。因此，癌症相关成纤维细胞的功能和分子表征是完全了解他们在肿瘤进展中的作用的必要条件。
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| Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation
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Author:
Date:
2013-09-05
[Abstract] A hallmark of cancer-associated inflammation is the infiltration of leukocytes into tumors, which is believed to be recruited by chemokines. Some infiltrating leukocytes such as macrophages often promote tumor growth by producing growth-inducing and angiogenic factors. Here, we described a method of isolating tumor-infiltrating leukocytes with Percoll density gradient, because Percoll possesses a low viscosity, a low osmolarity and no toxicity to cells. Different leukocyte populations are isolated based on their density and collected at the interface between 40% and 80% discontinuous Percoll gradient.
[摘要] 癌症相关炎症的标志是白细胞浸润到肿瘤中，据信这是由趋化因子募集的。 一些浸润性白细胞如巨噬细胞通常通过产生生长诱导和血管生成因子来促进肿瘤生长。 在这里，我们描述了一种用Percoll密度梯度分离肿瘤浸润白细胞的方法，因为Percoll具有低粘度，低渗透压和对细胞无毒性。 基于它们的密度分离不同的白细胞群体，并在40％和80％不连续的Percoll梯度之间的界面处收集。
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| Mouse Macrophage Differentiation by Induction with Macrophage Colony-Stimulating Factor
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Author:
Date:
2013-09-05
[Abstract] Macrophages are differentiated from circulating blood monocytes and act as tissue-resident professional phagocytes. Macrophages function in both innate and adaptive immune systems of vertebrate animals. The cytokine macrophage colony-stimulating factor (M-CSF) is essential for the proliferation and differentiation of monocytes. Here, we described a simple method to induce the differentiation of mouse bone marrow-derived myeloid precusor cells into macrophages in the presence of M-CSF.
[摘要] 巨噬细胞与循环血液单核细胞分化，并作为组织驻留专业吞噬细胞。 巨噬细胞在脊椎动物的先天和适应性免疫系统中起作用。 细胞因子巨噬细胞集落刺激因子（M-CSF）对于单核细胞的增殖和分化是必需的。 在这里，我们描述了一种简单的方法诱导小鼠骨髓衍生的骨髓前体细胞分化为巨噬细胞在M-CSF的存在。
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