Neurite Outgrowth Assay
|
Author:
Date:
2016-01-05
[Abstract] Neurite outgrowth in culture provides an easy way to determine the effects of a particular substrate or exogenous factor on neuron behavior. Dissociated neurons can be plated on a variety of substrates and the length of the longest neurite outgrowth can be compared. Here, we describe how to isolate and dissociate dorsal root ganglion (DRG) neurons, culture them on coverslips, and measure longest neurite outgrowth.
[摘要] 文化中的神经细胞生长提供了一种简单的方法来确定特定底物或外源因子对神经元行为的影响。 分离的神经元可以铺在各种底物上,并且可以比较最长的神经突生长的长度。 在这里,我们描述如何分离和解离背根神经节(DRG)神经元,将其培养在盖玻片上,并测量最长的神经突生长。
|
|
Protein Extraction, Acid Phosphatase Activity Assays, and Determination of Soluble Protein Concentration
|
Author:
Date:
2013-09-05
[Abstract] Acid phosphatases (APases) catalyze the hydrolysis of inorganic phosphate (Pi) from a broad range of Pi-monoesters with an acidic pH optimum. The liberated Pi is reassimilated into cellular metabolism via mitochondrial or chloroplastic ATP synthases of respiration or photosynthesis, respectively. Eukaryotic APases exist as a wide variety of tissue- and/or cellular compartment-specific isozymes that display marked differences in their physical and kinetic properties. Increases in intracellular (vacuolar) and secreted APase activities are useful biochemical markers of plant nutritional Pi deficiency. The protocols for protein extraction, APase activity determination and measurement of soluble protein concentration from plant tissues or cell suspension cultures are presented.
[摘要] 酸性磷酸酶(APase)催化来自具有酸性pH最佳值的宽范围的P 1 - 单酯的无机磷酸盐(P 1)的水解。 释放的Pi分别通过呼吸或光合作用的线粒体或叶绿体ATP合酶再吸收到细胞代谢中。 真核APase作为多种组织和/或细胞区室特异性同工酶存在,其在它们的物理和动力学性质上显示出显着的差异。 细胞内(液泡)和分泌的APase活性的增加是植物营养缺乏的有用的生物化学标记物。 提出了蛋白质提取,APase活性测定和来自植物组织或细胞悬浮培养物的可溶性蛋白质浓度的测定方案。
|
|