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Rabbit Immunoglobulins

兔免疫球蛋白

Company: Dako
Catalog#: D0306
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Colony Immunoblotting Assay for Detection of Bacterial Cell-surface or Extracellular Proteins
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2013-09-05
[Abstract]  This simple protocol describes how to detect antigens from agar-grown bacterial colonies transferred to nitrocellulose using specific antibodies. The protocol is well suitable for detection of bacterial proteins exposed on the cell surface or secreted to the extracellular space and it can be modified also for detection of intracellular proteins. The assay can distinguish bacterial clones with different expression rates (high, medium and low) from colonies that do not express target protein. We used this assay for screening of Mat fimbriae-producing Escherichia coli mutants obtained by mini-Tn5 transposon mutagenesis and immunomagnetic separation (Lehti et al., 2013). [摘要]  这个简单的协议描述如何使用特异性抗体检测从转移到硝酸纤维素的琼脂生长的细菌菌落的抗原。 该方案非常适合于检测暴露于细胞表面或分泌至细胞外空间的细菌蛋白,并且其还可以被修饰以用于检测细胞内蛋白。 该测定可以区分具有不同表达率(高,中和低)的细菌克隆与不表达靶蛋白的集落。 我们使用该测定法用于筛选通过mini-Tn5转座子诱变和免疫磁性分离获得的产生Mat菌毛的大肠杆菌突变体(Lehti等人,2013)。

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