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Author:
Date:
2013-09-05
[Abstract] The invasive ability of cancer cells is a crucial function for cancer metastasis and the surrounding microenvironment of cancer cells in living tissues is three-dimension (3D). Therefore, to establish an in vitro invasion assay in a 3D system to predict cancer invasive ability is valuable in the research for cancer metastasis. Here, we describe a 3D invasion assay for observing the morphology and comparing the invasive ability of cancer cells in artificial 3D environments (Yang et al., 2012). Collagen I gels are used to cover on the top of cancer cells attached on coverslip glass dish and medium containing FBS is added as a chemoattractant. After incubation for a suitable time, the cells are fixed and stained. The invasion index can be calculated and the morphology can ...
[摘要] 癌细胞的侵袭能力是癌症转移的关键功能，活组织中癌细胞的周围微环境是三维的（3D）。 因此，在3D系统中建立体外侵袭测定以预测癌症侵袭能力在癌症转移的研究中是有价值的。 在这里，我们描述了用于观察形态学并比较人造3D环境中癌细胞的侵袭能力的3D侵袭测定（Yang等人，2012）。 胶原I凝胶用于覆盖附着在盖玻片玻璃皿上的癌细胞的顶部，并且添加含有FBS的培养基作为化学引诱物。 在孵育合适的时间后，将细胞固定并染色。 可以计算入侵指数，并且可以用激光共聚焦显微镜成像形态
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