| Protocol for the Preparation of Arabidopsis Meiotic Chromosome Spreads and Fluorescent in situ Hybridization
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Author:
Date:
2014-04-20
[Abstract] This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the presence of defects in chromosome pairing and segregation. The protocol also describes how to perform fluorescent in situ hybridization experiments with a centromere probe used to label chromosomes.
[摘要] 该方案是为研究减数分裂染色体扩散而开发的以前方案的更详细的版本（Yang等人，2011;Bolaños-Villegas等人，2013）。 减数分裂染色体扩散可用于确定染色体配对和分离中缺陷的存在。 该方案还描述了如何使用用于标记染色体的着丝粒探针进行荧光原位杂交实验。
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| Isolation of Radiolabeled Poliovirus Particles from H1 HeLa Cells
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Author:
Date:
2013-08-20
[Abstract] The following protocol describes the isolation of radioactive viral and subviral particles from infected cells. This protocol has been written for isolation of poliovirus particles from H1 HeLa cells. Infection protocol and timing of [35S] methionine labeling and particle collection should be tailored to the virus of interest. Following isolation of the viral particles, the viral proteins present in these particles may be separated by gel electrophoresis and visualized by autoradiography.
[摘要] 以下方案描述了从感染的细胞中分离放射性病毒和亚病毒颗粒。 该协议是为了从H1 HeLa细胞分离脊髓灰质炎病毒颗粒而编写的。 [35]甲硫氨酸标记和颗粒收集的感染方案和时机应该针对感兴趣的病毒定制。 分离病毒颗粒后，可以通过凝胶电泳分离存在于这些颗粒中的病毒蛋白，并通过放射自显影进行显现。
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| Subcellular Fractionation of Mouse Brain Homogenates
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Author:
Date:
2013-08-05
[Abstract] This subcellular fractionation protocol is used for separation of cellular organelles based on their density. We have designed and optimized the protocol for separation of subcellular compartments of brain homogenates with focus on the localization and trafficking of transmembrane proteins, but we have also successfully used this protocol for fractionation of other types of tissue. The protocol has two major steps 1) preparation of homogenate from dissected tissue and 2) separation of organelles by centrifugation of homogenates using a continuous sucrose gradient.
[摘要] 这种亚细胞分馏方案用于根据细胞密度分离细胞器。 我们设计和优化了用于分离脑匀浆物的亚细胞区域的方案，重点是跨膜蛋白的定位和运输，但是我们也已经成功地使用该方案来分离其他类型的组织。 方案有两个主要步骤：1）从解剖组织制备匀浆物; 2）通过使用连续蔗糖梯度离心匀浆分离细胞器。
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