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His SpinTrap

他的SpinTrap

Company: GE Healthcare
Catalog#: 28-4013-53
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Probing Yeast Protein Microarrays for Protein-protein Interactions Using V5-epitope Tagged Fusion Protein Probes
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Date:
2012-03-05
[Abstract]  Protein microarray is probably the only technique currently available for systematic investigation of protein-protein interactions. This protocol describes an optimized method to probe yeast protein microarrays for protein-protein interactions using purified V5-epitope tagged fusion protein. It should also apply to any other proteins with appropriate modifications. [摘要]  蛋白质微阵列可能是目前唯一可用于系统调查蛋白质 - 蛋白质相互作用的技术。 该协议描述了使用纯化的V5-表位标记的融合蛋白探测蛋白质 - 蛋白质相互作用的酵母蛋白质微阵列的优化方法。 它也应适用于具有适当修饰的任何其他蛋白质。

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