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Luciferase Assay System, 10-Pack

荧光素酶测定系统,10包

Company: Promega
Catalog#: E1500
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Maize Embryo Transient Transformation by Particle Bombardment
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2013-08-20
[Abstract]  Particle bombardment has been shown to be a useful method to study gene promoter regulatory elements by transient transformation of maize embryos with different constructions of gene promoters fused to a gene reporter. DNA to transfer is coated to high density gold microparticles and introduced into cells when accelerated by a helium pulse. This method allows a first rapid approach, avoiding time consuming stable transformation of maize plants and also allows quantitative promoter expression analysis by a histochemical or fluorometric assay. [摘要]  已经证明粒子轰击是通过与基因报告基因融合的不同构建的基因启动子的玉米胚的瞬时转化来研究基因启动子调节元件的有用方法。 转移的DNA被涂覆到高密度的金微粒上,并通过氦脉冲加速时被引入细胞。 该方法允许第一快速方法,避免耗时的玉米植物的稳定转化,并且还允许通过组织化学或荧光测定进行定量启动子表达分析。

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