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Centrifuge

离心机

Company: Thermo Fisher Scientific
Catalog#: ST40
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Ex vivo Natural Killer Cell Cytotoxicity Assay
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Date:
2013-08-20
[Abstract]  Natural Killer (NK) cells are cytotoxic lymphocytes that constitute a major component of the innate immune system. Immunosurveillance of the host by NK cells for malignant and virally-infected cells results in direct cytotoxicity and the production of cytokines to enhance the immune response. This protocol will describe the “gold standard” chromium release assay for measuring the target cell killing capacity of NK cells. Key features of this cytotoxicity assay are that it is performed with sorted NK cells as the effectors and any Major Histocompatibility Class I (MHC-I)-low or deficient tumor cell line can be used as the target cells.
[摘要]  天然杀伤(NK)细胞是细胞毒性淋巴细胞,其构成先天性免疫系统的主要组分。 通过NK细胞对恶性和病毒感染的细胞的宿主的免疫监视导致直接的细胞毒性和细胞因子的产生,以增强免疫应答。 该方案将描述用于测量NK细胞的靶细胞杀伤能力的"金标准"铬释放测定。 该细胞毒性测定的主要特征是其使用分选的NK细胞作为效应物进行,任何主要组织相容性I类(MHC-1) - 低或缺陷的肿瘤细胞系可以用作靶细胞。

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