GoTaq® DNA Polymerase
Company: Promega
Catalog#: M3001
Product evaluation:
Targeted Gene Mutation in Rice Using a CRISPR-Cas9 System
Author:
Date:
2014-09-05
[Abstract]  RNA-guided genome editing (RGE) using bacterial type II cluster regularly interspaced short palindromic repeats (CRISPR)–associated nuclease (Cas) has emerged as a simple and versatile tool for genome editing in many organisms including plant and crop species. In RGE based on the Streptococcus pyogenes CRISPR-Cas9 system, the Cas9 nuclease is directed by a short single guide RNA (gRNA or sgRNA) to generate double-strand breaks (DSB) at the specific sites of chromosomal DNA, thereby ...

Comments
  • Bio-protocol’s mission is to improve research reproducibility. Please join our effort by sharing your experience on the reagents/equipment that you have used.
Similar products