T7 Endonuclease I
Company: New England Biolabs
Catalog#: M0302S
Product evaluation:
Generation of Targeted Knockout Mutants in Arabidopsis thaliana Using CRISPR/Cas9
Author:
Date:
2017-07-05
[Abstract]  The CRISPR/Cas9 system has emerged as a powerful tool for gene editing in plants and beyond. We have developed a plant vector system for targeted Cas9-dependent mutagenesis of genes in up to two different target sites in Arabidopsis thaliana. This protocol describes a simple 1-week cloning procedure for a single T-DNA vector containing the genes for Cas9 and sgRNAs, as well as the detection of induced mutations in planta. The procedure can likely be adapted for other ...

Targeted Gene Mutation in Rice Using a CRISPR-Cas9 System
Author:
Date:
2014-09-05
[Abstract]  RNA-guided genome editing (RGE) using bacterial type II cluster regularly interspaced short palindromic repeats (CRISPR)–associated nuclease (Cas) has emerged as a simple and versatile tool for genome editing in many organisms including plant and crop species. In RGE based on the Streptococcus pyogenes CRISPR-Cas9 system, the Cas9 nuclease is directed by a short single guide RNA (gRNA or sgRNA) to generate double-strand breaks (DSB) at the specific sites of chromosomal DNA, thereby ...

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