T4 DNA Ligase Reaction Buffer
Company: New England Biolabs
Catalog#: M0202S
Product evaluation:
Multiple Stepwise Gene Knockout Using CRISPR/Cas9 in Escherichia coli
Author:
Date:
2018-01-20
[Abstract]  With the recent implementation of the CRISPR/Cas9 technology as a standard tool for genome editing, laboratories all over the world are undergoing one of the biggest advancements in molecular biology since PCR. The key advantage of this method is its simplicity and universal applicability for species of any phylum. Of particular interest is the extensively studied Gram-negative bacterium Escherichia coli, as it is considered as the workhorse for both research and industrial purposes. ...

Selection of Genetically Modified Bacteriophages Using the CRISPR-Cas System
Author:
Date:
2017-08-05
[Abstract]  We present a CRISPR-Cas based technique for deleting genes from the T7 bacteriophage genome. A DNA fragment encoding homologous arms to the target gene to be deleted is first cloned into a plasmid. The T7 phage is then propagated in Escherichia coli harboring this plasmid. During this propagation, some phage genomes undergo homologous recombination with the plasmid, thus deleting the targeted gene. To select for these genomes, the CRISPR-Cas system is used to cleave non-edited genomes, ...

CRISPR/Cas9 Gene Editing in the Marine Diatom Phaeodactylum tricornutum
Author:
Date:
2017-08-05
[Abstract]  The establishment of the CRISPR/Cas9 technology in diatoms (Hopes et al., 2016; Nymark et al., 2016) enables a simple, inexpensive and effective way of introducing targeted alterations in the genomic DNA of this highly important group of eukaryotic phytoplankton. Diatoms are of interest as model microorganisms in a variety of areas ranging from oceanography to materials science, in nano- and environmental biotechnology, and are presently being investigated as a source of ...

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