HBSS, 10X, no Calcium, no Magnesium, no Phenol Red
Company: Thermo Fisher Scientific
Catalog#: 14185052
Product evaluation:
Generation of Chemically Induced Liver Progenitors (CLiPs) from Rat Adult Hepatocytes
[Abstract]  Primary mature hepatocytes (MHs) or their progenitor cells are candidate cell sources for cell transplantation therapy in severe liver diseases. However, stable culture of these cells or generation of equivalent cells from pluripotent stem cells has been limited. Using a cocktail of small molecules that we previously found useful in stable culture of multiple types of stem/progenitor cells, we recently established a novel method to generate bipotent liver progenitor cells, named chemically ...

Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons
[Abstract]  Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of mutant huntingtin (mHtt) secreted from Neuro2A cell line stably expressing mHtt and rat primary cortical neurons by Western blotting. Our protocol is based on reducing the amounts of BSA in the media while maintaining cell viability and secretory ...

Isolation of Primary Human Skeletal Muscle Cells
[Abstract]  Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing myogenicity and population expansion for subsequent in vitro study.

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