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HBSS, no calcium, no magnesium, no phenol red

HBSS,无钙,无镁,无酚红

Company: Thermo Fisher Scientific
Catalog#: 14175095
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Identification and Quantitation of Leukocyte Populations in Human Kidney Tissue by Multi-parameter Flow Cytometry
Author:
Date:
2018-08-20
[Abstract]  Inflammatory immune cells play direct pathological roles in cases of acute kidney injury (AKI) and chronic kidney disease (CKD). However, the identification and characterization of distinct populations of leukocytes in human kidney biopsies have been confounded by the limitations of immunohistochemical (IHC)-based techniques used to detect them. This methodology is not amenable to the combinations of multiple markers necessary to unequivocally define discrete immune cell populations. We have developed a multi-parameter, flow cytometric-based approach that addresses the need for panels of cell-specific markers in the identification of immune cell populations, allowing both the accurate detection and quantitation of leukocyte subpopulations from a single, clinical kidney biopsy specimen. In ... [摘要]  

Isolation of LYVE-1+ Endothelial Cells from Mouse Embryos
Author:
Date:
2018-08-05
[Abstract]  Lymphatic vessel endothelial hyaluronan receptor 1, or LYVE-1, is a type 1 integral membrane glycoprotein expressed by lymphatic endothelial cells (LECs). LYVE-1 is commonly used as a biological marker to visually distinguish developing lymphatic vessels from blood endothelial cells (arteries or veins). As our understanding of lymphatic biology is still lacking today, the need to isolate LECs apart from other endothelial cells has taken on greater importance. The following procedure describes a magnetic bead separation procedure for isolating LEC-rich populations of cells from developing mouse embryos. [摘要]  淋巴管内皮透明质酸受体1或LYVE-1是由淋巴管内皮细胞(LEC)表达的1型整合膜糖蛋白。 LYVE-1通常用作生物标记物,以在视觉上区分发育中的淋巴管与血液内皮细胞(动脉或静脉)。 由于我们今天仍然缺乏对淋巴生物学的理解,因此将LEC与其他内皮细胞分离的必要性变得更加重要。 以下程序描述了用于从发育中的小鼠胚胎中分离富含LEC的细胞群的磁珠分离程序。

【背景】淋巴脉管系统形成二次循环系统,其起到从组织排出细胞外液的作用,允许脂质的转运,并提供免疫细胞运输和运输功能。虽然我们对淋巴系统的理解在过去的几十年中迅速扩大,但与我们对动脉和静脉生物学的了解相比,它仍然缺乏。 LECs鉴定LYVE-1受体表达提供了区分淋巴组织的有用工具,但已知LYVE-1在其他组织中表达,包括肝和脾窦状细胞和胰腺外分泌和朗格汉斯细胞胰岛(Banerji et al。,1999)。由LYVE1基因编码,LYVE-1受体的生物学功能尚未确定,但除了跨内皮细胞的HA转运外,还有人建议参与肿瘤转移(Jackson,2003)。尽管LYVE-1在多种组织中表达,但它仍然是目前用于区分LEC与其他内皮细胞的常用标记物。该方法的目的是使用与LYVE-1抗体偶联的dynabeads以正选择方法从发育中的小鼠胚胎中分离LEC。

VLA-4 Affinity Assay for Murine Bone Marrow-derived Hematopoietic Stem Cells
Author:
Date:
2017-02-20
[Abstract]  Hematopoietic stem cells (HSCs) are defined by their functional ability to self-renew and to differentiate into all blood cell lineages. The majority of HSC reside in specific anatomical locations in the bone marrow (BM) microenvironment, in a quiescent non motile mode. Adhesion interactions between HSCs and their supporting BM microenvironment cells are critical for maintaining stem cell quiescence and protection from DNA damaging agents to prevent hematology failure and death. Multiple signaling proteins play a role in controlling retention and migration of bone marrow HSCs. Adhesion molecules are involved in both processes regulating hematopoiesis and stem- and progenitor-cell BM retention, migration and development. The mechanisms underlying the movement of stem cells from and to the ... [摘要]  造血干细胞(HSC)由其自我更新的功能能力定义,并分化成所有血细胞谱系。大多数HSC位于骨髓(BM)微环境中的特定解剖位置,处于静止非运动模式。 HSCs与其支持的BM微环境细胞之间的粘附相互作用对于维持干细胞静止和保护免受DNA损伤因子阻止血液学失败和死亡至关重要。多重信号蛋白在控制骨髓HSCs的保留和迁移中起重要作用。粘附分子参与调节造血和干细胞和祖细胞BM保留,迁移和发育的两个过程。干细胞从骨髓移动到骨髓的机制尚未完全阐明,仍然是深入研究的对象。一个重要的方面是修饰干细胞保留,迁移和发育所需的干细胞和祖细胞的粘附分子的表达和亲和力。粘附力通过粘附分子的表达,亲和力和亲合力来调节。亲和力调节与分子结合识别和结合强度有关。在这里,我们描述了在我们的研究中使用的体外 FACS测定来研究整联蛋白α4亚类β1亚单位的表达,亲和力和功能也称为VLA-4)用于小鼠骨髓保留EPCR +长期复制HSC(LT-HSC)(Gur-Cohen等人,2015)。背景整合素是介导细胞和细胞 - ...

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