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Author:
Date:
2013-07-05
[Abstract] Mesothelial cells (MCs) form a single squamous epithelial cell layer and cover the surfaces of the internal organs, as well as the walls of cavities. The isolation of MCs is of great importance to study their function and characteristics for the understanding of physiology and pathophysiology of the liver. Glycoprotein M6a (GPM6A) was originally identified as a cell surface protein expressed in neurons and recently its expression was reported in epicardium and liver MCs (Wu et al., 2001; Bochmann et al., 2010; Li et al., 2012). Here we describe a method to isolate MCs from the adult mouse liver with anti-GPM6A antibodies. Under the low glucose and serum concentration, primary MCs grow and form epithelial colonies (Figure 1).
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[摘要] 间皮细胞（MCs）形成单个鳞状上皮细胞层，并覆盖内部器官的表面，以及腔壁。 MC的分离对于研究它们的功能和特征对于理解肝脏的生理学和病理生理学是非常重要的。 糖蛋白M6a（GPM6A）最初被鉴定为在神经元中表达的细胞表面蛋白，并且最近在心外膜和肝脏MC中报道了其表达（Wu等人，2001; Bochmann等人， ，2010; Li等人，2012）。 在这里我们描述了一种方法从成年小鼠肝脏用抗GPM6A抗体分离MCs。 在低葡萄糖和血清浓度下，原代MC生长并形成上皮集落（图1）。
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