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Author:
Date:
2016-10-20
[Abstract] Protein kinase C-θ (PKC-θ), a member of the Ca2+-independent PKC subfamily of kinases, serves as a regulator of T cell activation by mediating the T cell antigen receptor (TCR)- and coreceptor CD28-induced activation of the transcription factors NF-κB and AP-1 and, to a lesser extent, NFAT, and, subsequently, interleukin 2 (IL-2) production and T cell proliferation. In T cells, TCR and CD28 stimulation-induced activation of PKC-θ is the integrated result of diacylglycerol-mediated membrane recruitment, GLK-mediated phosphorylation at activation loop, CD28, Lck, and sumoylation-mediated central immunological synapse localization (Wang et al., 2015; Monks et al., 1997; Kong et al., 2011; Isakov and Altman, 2012; Chuang et al., 2011). ...
[摘要] Sumoylation控制许多细胞过程。蛋白激酶C-θ（PKC-θ）是激酶的Ca 2+超家族PKC亚家族的成员，通过介导T细胞抗原受体（TCR）作为T细胞活化的调节剂， - 和共受体CD28诱导的转录因子NF-κB和AP-1的活化，以及较小程度的NFAT，以及随后的白细胞介素2（IL-2）产生和T细胞增殖。我们最近证明了TCR诱导的PKC-θ的sumoylation是其在T细胞中的功能所必需的（Wang等人，2015）。在这里我们描述分析TCR诱导的过表达或内源性PKC-θ的sumoylation的方法，这是通过免疫沉淀PKC-θ进行，然后用抗SUMO1抗体进行免疫印迹。
[背景] ...
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