| Generation of Polyclonal Specific Antibodies
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Author:
Date:
2013-06-05
[Abstract] Generation of antibodies specific for a protein of interest is a common method in many disciplines. This protocol details the steps in production of a polyclonal antibody in rabbits using a bacterially expressed fusion protein as an antigen. The protocol is generated based on data presented in Wirschell et al.(2013).
[摘要] 对感兴趣的蛋白质特异性的抗体的产生是许多学科中的常见方法。 该方案详述了使用细菌表达的融合蛋白作为抗原在兔中产生多克隆抗体的步骤。 该协议基于Wirschell等人（2013）中提供的数据生成。
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| Immunofluorescence (Especially for Cells Growing on a Coverglass)
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Author:
Date:
2012-02-20
[Abstract] If an antibody for your protein of interest is available, immunofluorscence is a useful method to detect the localization and relative abundance of the protein by using a fluorescence microscope. Immunofluoresence can be used in combination with other, non-antibody methods of fluorescence staining, for example, the use of DAPI to label DNA. This protocol describes setting up an immunofluorescence experiment using cells grown on a coverglass.
[摘要] 如果您感兴趣的蛋白质的抗体是可用的，免疫荧光是一种有用的方法，通过使用荧光显微镜检测蛋白质的本地化和相对丰度。 免疫荧光可以与其他非抗体荧光染色方法组合使用，例如，使用DAPI标记DNA。 该协议描述了使用生长在盖玻片上的细胞建立免疫荧光实验。
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| Immunofluorescence Assay for S Phase Entry Using BrdU Incorporation
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Author:
Date:
2012-02-05
[Abstract] Bromodeoxyuridine (BrdU) is thymidine analogue and can incorporated into the newly synthezised DNA of S-phase cells, the antibody specific for BrdU can then be used to detect the incorporated BrdU, thus indicating cells that were actively replicating their DNA and estimating for the percentage of cells in S-phase. The immunocytochemical detection of BrdU incorporated into DNA is a powerful tool to study the cytokinetics of normal and tumor cells. In vitro or in vivo labeling of tumor cells with BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.
[摘要] 溴脱氧尿苷（BrdU）是胸苷类似物并且可以并入S期细胞的新合成的DNA中，然后可以使用对BrdU特异性的抗体来检测掺入的BrdU，从而指示活跃地复制其DNA的细胞， 的S期细胞。 掺入DNA的BrdU的免疫细胞化学检测是研究正常和肿瘤细胞的细胞因子的有力工具。 在体外或体内用BrdU标记肿瘤细胞，随后用特异性抗BrdU单克隆抗体检测掺入的BrdU是一种准确和全面的方法， DNA合成。
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