Phorbol 12-myristate 13-acetate
Company: Sigma-Aldrich
Catalog#: 79346
Product evaluation:
Lymphocyte Isolation, Th17 Cell Differentiation, Activation, and Staining
[Abstract]  In vitro Th17 (α, β T helper cell which produce IL-17A, IL-17F and IL-22) differentiation has been routinely used for functional T cells studies. Here we describe a method for Th17 cell differentiation.

In vitro T Cell–DC and T Cell–T Cell Clustering Assays
[Abstract]  To get activated, T cells need to find their cognate antigen at the surface of an antigen-presenting cell (APC). Recognition of cognate antigen in the context of the MHC (Major histocompatibility complex) by the TCR (T-Cell Receptor) results in long lasting interactions between T cells and APCs. Subsequently, T cells form homotypic interactions with each other, which is seen as a hallmark of T cell activation. This protocol describes a method to analyze T-APC and T-T conjugation.

Intracellular Cytokine (INF-gamma) Staining Assay
[Abstract]  An intracellular cytokine (INF-gamma) staining assay is used to analyze the function of lymphocytes at the single cell level. By combining surface staining and intracellular cytokine staining, this assay can reveal the percentage of cytokine-releasing cells in a particular population, which cannot be obtained from an ELISpot assay.

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