|
Author:
Date:
2013-05-05
[Abstract] Transient gene expression via biolistic particle delivery is a widely used technique for gene functional analysis in plants. In this protocol we describe a modified single-cell transient expression assay through transformation with a particle inflow gun of the model PDS-1000/He system (Bio-Rad). This assay was originally optimized for analyzing cell death activity and disease resistance function of the barley MLA (mildew locus A) disease resistance proteins against the powdery mildew fungus, which can be further adopted for other purposes for other types of plant proteins and in some other plant species, including Arabidopsis thaliana.
[摘要] 通过生物射弹颗粒递送的瞬时基因表达是广泛使用的用于植物中基因功能分析的技术。 在该协议中，我们描述了通过用PDS-1000/He系统（Bio-Rad）的粒子流入枪转化的修饰的单细胞瞬时表达测定。 该测定最初被优化用于分析大麦MLA（霉菌基因座A）疾病抗性蛋白质对白粉菌真菌的细胞死亡活性和疾病抗性功能，其可以进一步用于其他目的用于其他类型的植物蛋白质和其他一些 植物物种，包括拟南芥（Arabidopsis thaliana）。
|