DMEM, High Glucose, Pyruvate, no Glutamine
Company: Thermo Fisher Scientific
Catalog#: 10313021
Product evaluation:
Coupling of HIV-1 gp120-derived Core Protein to Paramagnetic Beads and Adsorption Assays
Author:
Date:
2015-10-05
[Abstract]  Analysis of the functional activity in polyclonal serum following immunization of a complex protein or glycoprotein immunogen is a very important but tedious process. Fine mapping of epitope-specific antibodies is difficult when they are elicited at relatively low levels. In our recent study focused on developing an HIV-1 vaccine, we immunized rabbits with hyperglycosylated stable core immunogens, which were designed using high-resolution structural information to elicit antibodies against the ...

Longitudinal Bioluminescent Quantification of Three Dimensional Cell Growth
Author:
Date:
2013-12-05
[Abstract]  The use of three-dimensional (3D) cell culture systems is widely accepted as representing a more physiologically relevant means to propagate mammary epithelial and breast cancer cells. However, 3D cultures systems are plagued by several experimental and technical limitations as compared to their traditional 2D counterparts. For instance, quantifying the growth of mammary epithelial or breast cancer organoids longitudinally is particularly troublesome using standard [3H]thymidine or ...

Process and Analysis of Kidney Infiltrates by Flow Cytometry from Murine Lupus Nephritis
Author:
Date:
2012-05-05
[Abstract]  Methods for the isolation and characterization of mononuclear phagocytes from the kidneys of mice with SLE are essential to understand the patho-physiology of the disease. Activation of these cells is associated with the onset of clinical disease in mice and infiltration with these cells is associated with poor prognosis in humans.An analysis of the function of these cells should lead to a better understanding of the inflammatory processes that lead to renal impairment in SLE and other renal ...

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