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Histone H4ac (pan-acetyl) antibody (pAb)

组蛋白H4ac(pan-acetyl)抗体(pAb)

Company: Active Motif
Catalog#: 39925
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Western Analysis of Histone Modifications (Aspergillus nidulans)
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2013-04-05
[Abstract]  Western blotting allows for the specific detection of proteins and/or modifications of proteins by an antibody of interest. This protocol utilizes a crude nuclei extraction protocol for Aspergillus nidulans to enrich for histones and other nuclear proteins prior to gel electrophoresis. Post translational modifications of histones may then be easily detected. After electrophoresis, the selected antibodies are used to detect and quantify levels of the modifications of interest. [摘要]  蛋白质印迹允许由感兴趣的抗体特异性检测蛋白质和/或蛋白质的修饰。 该方案在凝胶电泳之前利用构巢曲霉的粗核提取方案来富集组蛋白和其它核蛋白。 然后可以容易地检测组蛋白的翻译后修饰。 电泳后,所选择的抗体用于检测和定量感兴趣的修饰的水平。

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