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Author:
Date:
2013-04-05
[Abstract] The reverse transcription (RT) reaction is a critical step in HIV-1 life cycle. It is very strongly regulated and the target of several restriction factors (TRIM5α, APOBECs, SAMHD1, etc.). The progress of reverse transcription can be followed by measuring viral DNA by quantitative PCR (qPCR). This method is sensitive enough to allow detection of low amounts of HIV-1 DNA in infected cells and discriminate between several types of reverse transcription intermediates (so called 《early》 and 《late》 RT products, 2 Long Terminal Repeat (LTR) circles, integrated DNA).
[摘要] 逆转录（RT）反应是HIV-1生命周期中的关键步骤。 它是非常强烈的调节和几个限制因素（TRIM5α，APOBECs，SAMHD1等）的目标。 逆转录的进程可以通过定量PCR（qPCR）测量病毒DNA。 该方法足够灵敏以允许在感染的细胞中检测到少量的HIV-1 DNA，并区分几种类型的逆转录中间体（所谓的"早期"和"晚期"RT产物，2个长末端重复（LTR） 整合DNA）。
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