| Qualitative in vivo Bioluminescence Imaging
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Author:
Date:
2018-09-20
[Abstract] Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ideal tool in exploring tumor growth and metastatic spread in real-time. This technique ensures the optimal use of laboratory animal resources, particularly the ethical principle of reduction in animal use, given its non-invasive nature, ensuring that ongoing biological processes can be studied over time in the same animal, without the need to euthanize groups of mice at specific time points. In this protocol, the luciferase imaging technique was developed to study the effect of co-inoculating pericytes (contractile, αSMA+ mesenchymal ...
[摘要] 生物发光成像（BLI）技术是一种在体内实验室动物上进行分子成像的先进方法。 这种强大的技术广泛应用于研究各种生物过程，是实时探索肿瘤生长和转移扩散的理想工具。 该技术确保实验室动物资源的最佳利用，特别是减少动物使用的伦理原则，考虑到其非侵入性，确保可以在同一动物中随时间研究正在进行的生物过程，而无需安乐死 小鼠在特定的时间点。 在该方案中，开发了荧光素酶成像技术以研究共同接种周细胞（收缩性，αSMA + 间充质干细胞样细胞，位于微血管内的细胞）对生长和转移性扩散的影响。 卵巢癌使用侵袭性卵巢癌细胞系-OVCAR-5-作为例子。
【背景】生物发光成像（BLI）的原理是基于相对简单的生化过程的发光特性，即，荧光素酶介导的分子底物荧光素氧化产生光。在癌症研究中，BLI是一种流行的工具（Contag et ...
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| Generation of Human iNKT Cell Lines
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Author:
Date:
2013-03-20
[Abstract] Natural killer T (NKT) cells comprise an important immunoregulatory T cell subset and express cell surface proteins characteristic of both natural killer cells and T cells. Invariant NKT (iNKT) cells are activated by lipid antigen presented in the context of CD1d molecules, in contrast to classic T cell subsets which recognize peptide antigens presented by MHC molecules. Following activation, iNKT cells rapidly secrete large amounts of cytokines and can lyse tumor cells and virally infected cells; however, iNKT cells are reduced in patients with autoimmune disease and cancer. The potential to characterize and investigate the prospective use of iNKT cells for therapeutic purposes has significantly increased with the ability to stimulate and expand human iNKT cells. In this protocol, we ...
[摘要] 自然杀伤T（NKT）细胞包含重要的免疫调节T细胞亚群，并表达天然杀伤细胞和T细胞特有的细胞表面蛋白。与识别由MHC分子呈递的肽抗原的经典T细胞亚群形成对比，不变NKT（iNKT）细胞被在CD1d分子的环境中存在的脂质抗原活化。激活后，iNKT细胞快速分泌大量细胞因子，并且可以裂解肿瘤细胞和病毒感染的细胞;然而，iNKT细胞在患有自身免疫性疾病和癌症的患者中减少。表征和研究iNKT细胞用于治疗目的的前瞻性用途的潜力随着刺激和扩增人iNKT细胞的能力而显着增加。在这个协议，我们描述一种生成和传播原代人类iNKT细胞的方法。具体地，从人外周血单核细胞（PBMC）分离初级iNKT细胞，然后在人IL-2存在下用照射的α-GalCer负载的自体未成熟树突细胞（DC）定期扩增。
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| Generation of Mouse iNKT Cell Lines
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Author:
Date:
2013-03-20
[Abstract] Natural killer T (NKT) cells bridge the innate and adaptive arms of the immune system, and manipulating their effector functions can have therapeutic significances in the treatment of autoimmunity, transplant biology, infectious disease and cancer. This important lymphocyte subset regulates the immune system through their potent cytokine production following the recognition of lipid antigen present in the context of the MHC class I-like CD1d molecule, in addition their ability to directly mediate cytotoxicity. Here, we describe a method of expanding mouse invariant NKT (iNKT) cell lines from mononuclear cells isolated from the thymus, spleen, or liver using bone marrow derived dendritic cells. These iNKT cell lines can be used study their co-signaling requirements, cytokine profiles ...
[摘要] 自然杀伤T（NKT）细胞桥接免疫系统的先天和适应性臂，并且操纵它们的效应子功能可以在治疗自身免疫，移植生物学，传染病和癌症中具有治疗意义。 这种重要的淋巴细胞亚群通过它们在识别存在于MHC I类CD1d分子的背景中的脂质抗原之后的有效细胞因子产生，以及它们直接介导细胞毒性的能力来调节免疫系统。 在这里，我们描述了扩增小鼠不变NKT（iNKT）细胞系从使用骨髓衍生的树突状细胞从胸腺，脾或肝分离的单核细胞的方法。 这些iNKT细胞系可用于研究其共信号要求，细胞因子谱和细胞毒性功能，这将大大增强我们对iNKT细胞生物学的知识。
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