| Organotypic Spinal Cord Slice Cultures and a Method to Detect Cell Proliferation in These Slices
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Author:
Date:
2016-10-05
[Abstract] In these culture models, the normal cytoarchitecture and local neuronal circuits of the spinal cord are preserved, offering a compromise between dissociated cell cultures and complete animal studies. The addition of 5-ethynyl-2’-deoxyuridine (EdU) to the culture medium allows for the detection of proliferating cells.
[摘要] 在这些文化模型中，保留了脊髓的正常细胞结构和局部神经元回路，提供了解离的细胞培养物和完全动物研究之间的折衷。 向培养基中加入5-乙炔基-2'-脱氧尿苷（EdU）允许检测增殖细胞。
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| Organotypic Slice Culture of Embryonic Brain Sections
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Author:
Date:
2013-02-05
[Abstract] This technique will allow using brain slices to study several aspects of cortical development (i.e. neurogenesis), as well as neuronal differentiation (i.e. neuronal migration, axon and dendrite formation) in situ. This protocol is suitable for various embryonic stages (Calderon de Anda et al., 2010; Ge et al., 2010).
[摘要] 这种技术将允许使用脑切片研究皮质发育（即神经发生）以及神经元分化（即神经元迁移，轴突和树突形成）的几个方面。 >原位。 该方案适用于各种胚胎阶段（Calderon de Anda等人，2010; Ge等人，2010）。
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