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Sodium chloride physiological solution

氯化钠生理溶液

Company: Sigma-Aldrich
Catalog#: 7982
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Isolation, Purification, and Culture of Primary Murine Microglia Cells
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2013-01-05
[Abstract]  The following is a detailed protocol for the isolation, purification and culture of murine brain microglia cells using neutral enzyme digestion and shaking. The protocol below is designed to isolate and culture a large number of purified inactivated microglia cells. Neutral enzyme digestion allows for minimal cellular damage and a higher cell recovery compared to other mechanical dissociative methods such as mincing. Cells cultured using this method will display the size and morphological features of microglia cells. [摘要]  以下是使用中性酶消化和摇动的鼠脑小胶质细胞的分离,纯化和培养的详细方案。 以下方案设计用于分离和培养大量纯化的灭活的小神经胶质细胞。 与其他机械解离方法例如切碎相比,中性酶消化允许最小的细胞损伤和更高的细胞回收率。 使用这种方法培养的细胞将显示小神经胶质细胞的大小和形态特征。

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