Millex-AP Syringe Filter Unit

Millex-AP注射器过滤器单元

Company: EMD Millipore

Catalog#: SLAP02550

Preparation and Purification of β-1,3-glucan-Linked Candida glabrata Cell Wall Proteases by Ion-Exchange Chromatography, Gel Filtration, and MDPF-Gelatin-Zymography Assay

Pirjo Pärnänen, Timo Sorsa, Taina Tervahartiala and Pirjo Nikula-Ijäs

Published online: 2024-03-20

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Purification of 38kDa Antigen of Mycobacterium tuberculosis by Two Dimensional Preparative Electrophoresis

Author:

Uma Devi Ranganathan,

Date:

2012-12-20

[Abstract] Serodiagnosis of tuberculosis using purified native antigens is one of the approach for the early detection of TB. This protocol gives the details for growing Mycobacterium tuberculosis in liquid culture and one proven way to get a good yield of the total secreted antigens in the culture supernatant. In addition the procedure for purification of the 38 kDa antigen from the total culture filtrate antigens is also explained. 38 kDa is one of the specific antigen for Mycobacterium tuberculosis complex and in this particular study it was used as one of the antigens for serodiagnosis of different category of tuberculosis patients. [摘要] 使用纯化的天然抗原的结核病的血清诊断是早期检测TB的方法之一。该方案提供了在液体培养物中生长结核分枝杆菌的细节，以及一种在培养物上清液中获得总分泌抗原的良好产量的证明的方法。此外，还解释了从总培养物滤液抗原中纯化38kDa抗原的方法。 38kDa是结核分枝杆菌复合物的特异性抗原之一，并且在该特定研究中，其用作不同类别的结核病患者的血清诊断的抗原之一。

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Millex-AP Syringe Filter Unit

Millex-AP注射器过滤器单元

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